Modulation of the combinatorial code of odorant receptor response patterns in odorant mixtures.
G protein-coupled receptors
Olfaction
Olfactory coding
Olfactory sensory neurons
Journal
Molecular and cellular neurosciences
ISSN: 1095-9327
Titre abrégé: Mol Cell Neurosci
Pays: United States
ID NLM: 9100095
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
21
10
2019
revised:
10
02
2020
accepted:
11
02
2020
pubmed:
18
2
2020
medline:
2
1
2021
entrez:
17
2
2020
Statut:
ppublish
Résumé
The perception of odors relies on combinatorial codes consisting of odorant receptor (OR) response patterns to encode odor identity. Modulation of these patterns by odorant interactions at ORs potentially explains several olfactory phenomena: mixture suppression, unpredictable sensory outcomes, and the perception of odorant mixtures as unique objects. We determined OR response patterns to 4 odorants and 3 binary mixtures in vivo in mice, identifying 30 responsive ORs. These patterns typically had a few strongly responsive ORs and a greater number of weakly responsive ORs. ORs responsive to an odorant were often unrelated sequences distributed across several OR subfamilies. Mixture responses predicted pharmacological interactions between odorants, which were tested in vitro by heterologous expression of ORs in cultured cells, providing independent evidence confirming odorant agonists for 13 ORs and identifying both suppressive and additive effects. This included 11 instances of antagonism of ORs by an odorant, 1 instance of additive responses to a binary mixture, 1 instance of suppression of a strong agonist by a weak agonist, and the discovery of an inverse agonist for an OR. Interactions between odorants at ORs are common even when the odorants are not known to interact perceptually in humans, and in some cases interactions at mouse ORs correlate with the ability of humans to perceive an odorant in a mixture.
Identifiants
pubmed: 32061665
pii: S1044-7431(19)30296-9
doi: 10.1016/j.mcn.2020.103469
pmc: PMC7138139
mid: NIHMS1576613
pii:
doi:
Substances chimiques
Aldehydes
0
Lactones
0
Pentanols
0
Receptors, Odorant
0
undecanal
B6P0A9PSHN
bourgeonal
H3XVS6E70V
isoamyl acetate
Z135787824
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
103469Subventions
Organisme : NIDCD NIH HHS
ID : K99 DC018333
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC014423
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC014468
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC016224
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest T.S.M. has an equity interest in a company based on technologies used to measure responses to odors. H.M. receives royalties from Chemcom.
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