Alpha- and gamma-tocopherol levels in human semen and their potential functional implications.
GC-MS analysis
male fertility
oxidative stress
sperm cryopreservation
vitamin E
Journal
Andrologia
ISSN: 1439-0272
Titre abrégé: Andrologia
Pays: Germany
ID NLM: 0423506
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
16
12
2019
revised:
07
01
2020
accepted:
11
01
2020
pubmed:
18
2
2020
medline:
14
1
2021
entrez:
18
2
2020
Statut:
ppublish
Résumé
Vitamin E is a potent antioxidant that has been considered involved in fertility, but studies have mostly focused on α-tocopherol. Our study aimed at measuring, by an isotope dilution gas chromatography-mass spectrometry method, α- and γ-tocopherol concentration in human semen in a large and well-characterised population (134 men with different semen parameters and in varicocele patients), as well as their potential role in male fertility. We carried out freeze/thaw experiments in 15 samples with the two isomers in the cryoprotective medium. Moreover, our study included 10 subjects supplemented in vivo with α-tocopherol for 90 days. In seminal plasma, γ-tocopherol concentration was significantly lower in the varicocele group than in the normozoospermic group. We observed that γ-tocopherol, supplemented to cryopreservation medium, induced a higher post-thaw human sperm viability and motility than α-tocopherol. The results of in vivo α-tocopherol supplementation showed a decrease in γ-tocopherol concentration with increasing α-tocopherol level in blood. This is the first report related to γ-tocopherol distribution in human semen analysed by gas chromatography-mass spectrometry. γ-tocopherol would not seem to be related to semen parameters but to cellular oxidative condition. This tocopherol may contribute to human health in a yet unexplored way.
Substances chimiques
gamma-Tocopherol
8EF1Z1238F
alpha-Tocopherol
H4N855PNZ1
Types de publication
Clinical Trial
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13543Subventions
Organisme : Sapienza University of Rome
ID : C26A15M78W
Informations de copyright
© 2020 Blackwell Verlag GmbH.
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