Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner.
STED nanoscopy
crista junction
cristae
membrane dynamics
membrane potential
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
04 03 2020
04 03 2020
Historique:
received:
28
11
2019
revised:
13
01
2020
accepted:
14
01
2020
pubmed:
19
2
2020
medline:
28
4
2021
entrez:
19
2
2020
Statut:
ppublish
Résumé
The mitochondrial inner membrane can reshape under different physiological conditions. How, at which frequency this occurs in living cells, and the molecular players involved are unknown. Here, we show using state-of-the-art live-cell stimulated emission depletion (STED) super-resolution nanoscopy that neighbouring crista junctions (CJs) dynamically appose and separate from each other in a reversible and balanced manner in human cells. Staining of cristae membranes (CM), using various protein markers or two lipophilic inner membrane-specific dyes, further revealed that cristae undergo continuous cycles of membrane remodelling. These events are accompanied by fluctuations of the membrane potential within distinct cristae over time. Both CJ and CM dynamics depended on MIC13 and occurred at similar timescales in the range of seconds. Our data further suggest that MIC60 acts as a docking platform promoting CJ and contact site formation. Overall, by employing advanced imaging techniques including fluorescence recovery after photobleaching (FRAP), single-particle tracking (SPT), live-cell STED and high-resolution Airyscan microscopy, we propose a model of CJ dynamics being mechanistically linked to CM remodelling representing cristae membrane fission and fusion events occurring within individual mitochondria.
Identifiants
pubmed: 32067344
doi: 10.15252/embr.201949776
pmc: PMC7054676
doi:
Substances chimiques
Mitochondrial Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e49776Subventions
Organisme : Research Committee of the Medical faculty of Heinrich Heine University Düsseldorf
ID : FoKo-37/2015
Pays : International
Organisme : Research Committee of the Medical faculty of Heinrich Heine University Düsseldorf
ID : Foko-02/2015
Pays : International
Organisme : Deutsche Forschungsgemeinschaft
ID : RE 1575/2-1
Pays : International
Organisme : Deutsche Forschungsgemeinschaft
ID : Project-ID 267205415-SFB 1208 project B12
Pays : International
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB 974 Project B09
Pays : International
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB 1208 Project Z02
Pays : International
Organisme : Deutsche Forschungsgemeinschaft
ID : WE 5343/1-1
Pays : International
Informations de copyright
© 2020 The Authors. Published under the terms of the CC BY NC ND 4.0 license.
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