Cytomegalovirus (CMV) Shedding in French Day-Care Centers: A Nationwide Study of Epidemiology, Risk Factors, Centers' Practices, and Parents' Awareness of CMV.


Journal

Journal of the Pediatric Infectious Diseases Society
ISSN: 2048-7207
Titre abrégé: J Pediatric Infect Dis Soc
Pays: England
ID NLM: 101586049

Informations de publication

Date de publication:
31 Dec 2020
Historique:
received: 01 08 2019
accepted: 12 02 2020
pubmed: 19 2 2020
medline: 19 8 2021
entrez: 19 2 2020
Statut: ppublish

Résumé

Congenital cytomegalovirus (CMV) remains an important healthcare burden, resulting from primary or secondary infection in pregnant women. Exposure to young children's saliva is a major risk factor, as prevalence of CMV shedding can reach 34%. This cross-sectional, multicenter, nationwide study was conducted in randomly selected day care centers (DCCs), and complemented with a survey among parents and DCCs. All children aged >3 months were eligible. The study measured the CMV shedding prevalence in children's saliva and described CMV genotypes epidemiology. The risk factors for CMV shedding and high viral load were evaluated using multivariable models. A total of 93 DCCs participated. Among the 1770 enrolled children with evaluable samples, the CMV shedding prevalence was 40% (713/1770, 95% confidence interval, 34.6-46.1), independently associated with children aged between 12 and 18 months, history of CMV infection in ≥1 parents, a mid-level income. Prevalence increased with DCC staff workload and attending children number. Viral load was ≥5 log-copies CMV/mL in 48% (342/713). Risk factors for higher viral load included children aged between 12 and 18 months, and still being breastfed. The most frequent genotype combinations were gB1-gN4c-gH2 (6.9%), gB1-gN2-gH2 (6.3%), gB4a-gN3a-gH1 (6.3%), and gB1-gN3b-gH2 (5,7%). CMV awareness was low in parents: their serological status was unknown by 72% of mothers and 82% of fathers. Only 41% knew something about CMV. CMV shedding was independently associated with risk factors related to the children, family and DCC. Some of these risk factors may influence prevention strategies, including through an improved information provided to parents. NCT01704222.

Sections du résumé

BACKGROUND BACKGROUND
Congenital cytomegalovirus (CMV) remains an important healthcare burden, resulting from primary or secondary infection in pregnant women. Exposure to young children's saliva is a major risk factor, as prevalence of CMV shedding can reach 34%.
METHODS METHODS
This cross-sectional, multicenter, nationwide study was conducted in randomly selected day care centers (DCCs), and complemented with a survey among parents and DCCs. All children aged >3 months were eligible. The study measured the CMV shedding prevalence in children's saliva and described CMV genotypes epidemiology. The risk factors for CMV shedding and high viral load were evaluated using multivariable models.
RESULTS RESULTS
A total of 93 DCCs participated. Among the 1770 enrolled children with evaluable samples, the CMV shedding prevalence was 40% (713/1770, 95% confidence interval, 34.6-46.1), independently associated with children aged between 12 and 18 months, history of CMV infection in ≥1 parents, a mid-level income. Prevalence increased with DCC staff workload and attending children number. Viral load was ≥5 log-copies CMV/mL in 48% (342/713). Risk factors for higher viral load included children aged between 12 and 18 months, and still being breastfed. The most frequent genotype combinations were gB1-gN4c-gH2 (6.9%), gB1-gN2-gH2 (6.3%), gB4a-gN3a-gH1 (6.3%), and gB1-gN3b-gH2 (5,7%). CMV awareness was low in parents: their serological status was unknown by 72% of mothers and 82% of fathers. Only 41% knew something about CMV.
CONCLUSIONS CONCLUSIONS
CMV shedding was independently associated with risk factors related to the children, family and DCC. Some of these risk factors may influence prevention strategies, including through an improved information provided to parents.
CLINICAL TRIALS REGISTRATION BACKGROUND
NCT01704222.

Identifiants

pubmed: 32068854
pii: 5739998
doi: 10.1093/jpids/piz097
doi:

Banques de données

ClinicalTrials.gov
['NCT01704222']

Types de publication

Journal Article Multicenter Study

Langues

eng

Sous-ensembles de citation

IM

Pagination

686-694

Informations de copyright

© The Author(s) 2020. Published by Oxford University Press on behalf of The Journal of the Pediatric Infectious Diseases Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Auteurs

Sophie Alain (S)

National Reference Center for Herpesviruses, Limoges, France.
Inserm U1092 University Limoges, Limoges, France.
Bacteriology, Virology, Hygiene Department, CHU Limoges, Limoges, France.

Françoise Garnier-Geoffroy (F)

National Reference Center for Herpesviruses, Limoges, France.
Inserm U1092 University Limoges, Limoges, France.
Bacteriology, Virology, Hygiene Department, CHU Limoges, Limoges, France.

Anaïs Labrunie (A)

Centre for Methodology and Data Management (Cebimer Department), CHU Limoges, Limoges, France.

Amélie Montané (A)

Paediatrics Department, CHU Limoges, France.

Benoit Marin (B)

Centre for Methodology and Data Management (Cebimer Department), CHU Limoges, Limoges, France.
General Directorate for Health, Paris, France.

Martine Gatet (M)

Inserm U1092 University Limoges, Limoges, France.

Jérôme Grosjean (J)

National Reference Center for Herpesviruses, Limoges, France.
Bacteriology, Virology, Hygiene Department, CHU Limoges, Limoges, France.
Biology Department, Chambery Hospital, Chambery, France.

Véronique Dufour (V)

Families and Early Childhood Directorate, Infantile Maternal Protection Department, Paris, France.

Mathilde Saugeras (M)

Center of Clinical Investigation Department, CHU Limoges, Limoges, France.

Deborah Postil (D)

Centre for Methodology and Data Management (Cebimer Department), CHU Limoges, Limoges, France.

Sébastien Hantz (S)

National Reference Center for Herpesviruses, Limoges, France.
Inserm U1092 University Limoges, Limoges, France.
Bacteriology, Virology, Hygiene Department, CHU Limoges, Limoges, France.

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