Amperometric Bioplatforms To Detect Regional DNA Methylation with Single-Base Sensitivity.
Journal
Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536
Informations de publication
Date de publication:
07 04 2020
07 04 2020
Historique:
pubmed:
20
2
2020
medline:
21
1
2021
entrez:
20
2
2020
Statut:
ppublish
Résumé
This work reports the first bioplatform able to determine electrochemically 5-hydroxymethylcytosine (5-hmC) methylation events at localized sites and single-base sensitivity. The described bioplatform relies on a specific antibody (anti-5-hmC), further conjugated with commercial bioreagents loaded with multiple horseradish peroxidase (HRP) molecules, recognizing the epimark in a target DNA, captured through hybridization onto streptavidin-magnetic microbeads (Strep-MBs) modified with a complementary DNA capture probe. The electrochemical detection is performed by amperometry (-0.20 V vs Ag pseudoreference electrode) at disposable screen-printed carbon electrodes (SPCEs) in the presence of H
Identifiants
pubmed: 32073832
doi: 10.1021/acs.analchem.0c00628
doi:
Substances chimiques
5-hydroxymethylcytosine
1123-95-1
5-Methylcytosine
6R795CQT4H
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM