The African Swine Fever Virus Transcriptome.
African Swine Fever
/ prevention & control
African Swine Fever Virus
/ genetics
Amino Acid Sequence
Animals
Genome, Viral
Hemorrhagic Fevers, Viral
/ virology
Sus scrofa
/ virology
Swine
/ virology
Transcription Termination, Genetic
Transcription, Genetic
/ genetics
Transcriptional Activation
/ genetics
Transcriptome
/ genetics
Viral Proteins
/ genetics
African swine fever virus
NCLDV
RNA polymerases
RNA-seq
gene expression
promoters
transcription
transcription start site
virology
zoonotic infections
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
16 04 2020
16 04 2020
Historique:
received:
22
01
2020
accepted:
04
02
2020
pubmed:
23
2
2020
medline:
21
10
2020
entrez:
21
2
2020
Statut:
epublish
Résumé
African swine fever virus (ASFV) causes hemorrhagic fever in domestic pigs, presenting the biggest global threat to animal farming in recorded history. Despite the importance of ASFV, little is known about the mechanisms and regulation of ASFV transcription. Using RNA sequencing methods, we have determined total RNA abundance, transcription start sites, and transcription termination sites at single-nucleotide resolution. This allowed us to characterize DNA consensus motifs of early and late ASFV core promoters, as well as a polythymidylate sequence determinant for transcription termination. Our results demonstrate that ASFV utilizes alternative transcription start sites between early and late stages of infection and that ASFV RNA polymerase (RNAP) undergoes promoter-proximal transcript slippage at 5' ends of transcription units, adding quasitemplated AU- and AUAU-5' extensions to mRNAs. Here, we present the first much-needed genome-wide transcriptome study that provides unique insight into ASFV transcription and serves as a resource to aid future functional analyses of ASFV genes which are essential to combat this devastating disease.
Identifiants
pubmed: 32075923
pii: JVI.00119-20
doi: 10.1128/JVI.00119-20
pmc: PMC7163114
pii:
doi:
Substances chimiques
Viral Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Wellcome Trust
ID : WT 108877/B/15/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : WT 207446/Z/17/Z
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/0007030
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007034
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/0007031
Pays : United Kingdom
Organisme : Wellcome Trust
ID : WT 095598/Z/11/Z
Pays : United Kingdom
Informations de copyright
Copyright © 2020 Cackett et al.
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