Transplantation of iPSC-TM stimulates division of trabecular meshwork cells in human eyes.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
19 02 2020
19 02 2020
Historique:
received:
12
07
2019
accepted:
03
02
2020
entrez:
21
2
2020
pubmed:
23
2
2020
medline:
13
11
2020
Statut:
epublish
Résumé
The trabecular meshwork's (TM) physiological role is to maintain normal intraocular pressure by regulating aqueous humor outflow. With age, and particularly in eyes with primary open angle glaucoma, the number of cells residing within the TM is markedly decreased and the function of the tissue is compromised. Here we evaluate if transplantation of induced pluripotent stem cell derived TM like cells (iPSC-TM) restores TM cellularity and function in human eyes obtained from older human donors. Human iPSC were differentiated into iPSC-TM and compared to primary TM cells by RNAseq. iPSC-TM were then injected into the anterior segments of human eyes maintained in perfusion culture. Seven and 14 days eyes after injection eyes that received iPSC-TM contained significantly more cells in the TM. Fewer than 1% of all cells appeared to be iPSC-TM, but significantly more cells in these eyes were immunopositive for Ki 67 and incorporated BrdU. Our study demonstrates that transplantation iPSC-TM stimulates proliferation of endogenous TM cells in perfusion cultured human eyes from aged donors. These data, in concert with our previous findings in animal models, suggest that functional regeneration of the TM may be possible in human eyes with primary open angle glaucoma.
Identifiants
pubmed: 32076077
doi: 10.1038/s41598-020-59941-0
pii: 10.1038/s41598-020-59941-0
pmc: PMC7031365
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
2905Subventions
Organisme : RRD VA
ID : I01 RX001163
Pays : United States
Organisme : RRD VA
ID : I01 RX002860
Pays : United States
Organisme : RRD VA
ID : I50 RX003002
Pays : United States
Organisme : NEI NIH HHS
ID : P30 EY025580
Pays : United States
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