Steroids Inhibit Eosinophil Accumulation and Downregulate Hematopoietic Chemotaxic Prostaglandin D


Journal

Ear, nose, & throat journal
ISSN: 1942-7522
Titre abrégé: Ear Nose Throat J
Pays: United States
ID NLM: 7701817

Informations de publication

Date de publication:
Sep 2021
Historique:
pubmed: 23 2 2020
medline: 17 12 2021
entrez: 21 2 2020
Statut: ppublish

Résumé

Aspirin-exacerbated respiratory disease (AERD) is characterized by eosinophilic rhinosinusitis, nasal polyposis, aspirin sensitivity, and asthma. Among 37 patients undergoing endoscopic sinus surgery, 28 received systemic steroids preoperatively. Nasal polyps were harvested from all 37 patients. After routine processing of paraffin sections, immunohistochemistry was performed using specific antibodies for HPGDS, eosinophil peroxidase (EPX), and DP2. Expression of HPGDS, DP2, and EPX by eosinophils was higher and more frequent in patients with non-preoperative steroid therapy. Likewise, HPGDS and DP2 were highly expressed in activated eosinophils in the nasal polyps, but not in normal eosinophils. This study provides clear evidence that systemic steroid therapy inhibits eosinophil activation and decreases HPGDS and DP2 expression in patients with AERD, indicating a reduction in prostaglandin D

Sections du résumé

BACKGROUND BACKGROUND
Aspirin-exacerbated respiratory disease (AERD) is characterized by eosinophilic rhinosinusitis, nasal polyposis, aspirin sensitivity, and asthma.
MATERIALS AND METHODS METHODS
Among 37 patients undergoing endoscopic sinus surgery, 28 received systemic steroids preoperatively. Nasal polyps were harvested from all 37 patients. After routine processing of paraffin sections, immunohistochemistry was performed using specific antibodies for HPGDS, eosinophil peroxidase (EPX), and DP2.
RESULTS RESULTS
Expression of HPGDS, DP2, and EPX by eosinophils was higher and more frequent in patients with non-preoperative steroid therapy. Likewise, HPGDS and DP2 were highly expressed in activated eosinophils in the nasal polyps, but not in normal eosinophils.
CONCLUSION AND SIGNIFICANCE CONCLUSIONS
This study provides clear evidence that systemic steroid therapy inhibits eosinophil activation and decreases HPGDS and DP2 expression in patients with AERD, indicating a reduction in prostaglandin D

Identifiants

pubmed: 32077309
doi: 10.1177/0145561320902858
doi:

Substances chimiques

Adrenal Cortex Hormones 0
Cyclooxygenase Inhibitors 0
Receptors, Immunologic 0
Receptors, Prostaglandin 0
Eosinophil Peroxidase EC 1.11.1.-
Intramolecular Oxidoreductases EC 5.3.-
PTGDS protein, human EC 5.3.-
prostaglandin D2 receptor XZF106QU24

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

738S-745S

Auteurs

Norio Suzuki (N)

Department of Otolaryngology, Head and Neck Surgery, 13010Osaka Medical College, Osaka, Japan.

Elizabeth P Ko-Mitamura (EP)

Department of Animal Radiology and Graduate School of Agriculture and Life Sciences, 13143The University of Tokyo, Tokyo, Japan.

Takaki Inui (T)

Department of Otolaryngology, Head and Neck Surgery, 13010Osaka Medical College, Osaka, Japan.

Tetsuya Terada (T)

Department of Otolaryngology, Head and Neck Surgery, 13010Osaka Medical College, Osaka, Japan.

Kenji Dejima (K)

Department of Otolaryngology, 38334Kyoto Second Red Cross Hospital, Kyoto, Japan.

Nanae Nagata (N)

Department of Animal Radiology and Graduate School of Agriculture and Life Sciences, 13143The University of Tokyo, Tokyo, Japan.

Yoshihiro Urade (Y)

26782The University of Tokyo Hospital, Tokyo, Japan.

Ryo Kawata (R)

Department of Otolaryngology, Head and Neck Surgery, 13010Osaka Medical College, Osaka, Japan.

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Classifications MeSH