In vivo fluorescence-based characterization of cytochrome P450 activity during embryonic development of zebrafish (Danio rerio).
7-Methoxycoumarin-O-demethylation
Biotransformation
Cytochrome P450 monooxygenases
Xenobiotic metabolism
Zebrafish embryo
Journal
Ecotoxicology and environmental safety
ISSN: 1090-2414
Titre abrégé: Ecotoxicol Environ Saf
Pays: Netherlands
ID NLM: 7805381
Informations de publication
Date de publication:
01 Apr 2020
01 Apr 2020
Historique:
received:
12
11
2019
revised:
03
02
2020
accepted:
12
02
2020
pubmed:
23
2
2020
medline:
21
5
2020
entrez:
21
2
2020
Statut:
ppublish
Résumé
Zebrafish (Danio rerio) early life-stages are increasingly gaining attention as an alternative model in both human and environmental toxicology. Whereas there is amble knowledge about the transcription of various cytochrome P450 isoforms, the level of information about functional implications is still limited. This study investigated the development of CYP2-dependent 7-methoxycoumarin-O-demethylase (MCOD) activity throughout the early zebrafish development from 5 to 118 h post-fertilization (hpf) via confocal laser scanning microscopy. Results demonstrate that zebrafish embryos exhibit constitutive MCOD activity from as early as 5.5 hpf. Characteristic spatiotemporal patterns were documented with MCOD activities localized in several tissues and organs, namely the cardiovascular system, the brain, the digestive system, and the urinary tract. The study thereby contributes to a better understanding of the development and functional role of CYP enzymes in zebrafish early life-stages.
Identifiants
pubmed: 32078841
pii: S0147-6513(20)30169-X
doi: 10.1016/j.ecoenv.2020.110330
pii:
doi:
Substances chimiques
7-methoxycoumarin-O-demethylase
EC 1.-
Oxidoreductases, O-Demethylating
EC 1.-
Cytochrome P450 Family 2
EC 1.14.14.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
110330Informations de copyright
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