Beyond oil degradation: enzymatic potential of Alcanivorax to degrade natural and synthetic polyesters.
Journal
Environmental microbiology
ISSN: 1462-2920
Titre abrégé: Environ Microbiol
Pays: England
ID NLM: 100883692
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
16
12
2019
accepted:
18
02
2020
pubmed:
23
2
2020
medline:
15
12
2020
entrez:
21
2
2020
Statut:
ppublish
Résumé
Pristine marine environments are highly oligotrophic ecosystems populated by well-established specialized microbial communities. Nevertheless, during oil spills, low-abundant hydrocarbonoclastic bacteria bloom and rapidly prevail over the marine microbiota. The genus Alcanivorax is one of the most abundant and well-studied organisms for oil degradation. While highly successful under polluted conditions due to its specialized oil-degrading metabolism, it is unknown how they persist in these environments during pristine conditions. Here, we show that part of the Alcanivorax genus, as well as oils, has an enormous potential for biodegrading aliphatic polyesters thanks to a unique and abundantly secreted alpha/beta hydrolase. The heterologous overexpression of this esterase proved a remarkable ability to hydrolyse both natural and synthetic polyesters. Our findings contribute to (i) better understand the ecology of Alcanivorax in its natural environment, where natural polyesters such as polyhydroxyalkanoates (PHA) are produced by a large fraction of the community and, hence, an accessible source of carbon and energy used by the organism in order to persist, (ii) highlight the potential of Alcanivorax to clear marine environments from polyester materials of anthropogenic origin as well as oils, and (iii) the discovery of a new versatile esterase with a high biotechnological potential.
Identifiants
pubmed: 32079039
doi: 10.1111/1462-2920.14947
pmc: PMC7187450
doi:
Substances chimiques
Oils
0
Polyesters
0
Polyhydroxyalkanoates
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1356-1369Subventions
Organisme : Consejo Nacional de Innovación, Ciencia y Tecnología
ID : 72160583
Pays : International
Organisme : European Social Fund
ID : RYC-2017-22452
Pays : International
Organisme : Ministerio de Economía y Competitividad
ID : CTM2015-70180-R
Pays : International
Organisme : Natural Environment Research Council
ID : NE/K009044/1
Pays : International
Organisme : Natural Environment Research Council
ID : NE/S005501/1
Pays : International
Organisme : Ministry of Science, Innovation and Universities
Pays : International
Informations de copyright
© 2020 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.
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