Inhibition of Porphyromonas gulae and periodontal disease in dogs by a combination of clindamycin and interferon alpha.
Animals
Bacteroidaceae Infections
/ drug therapy
Cell Line
Clindamycin
/ administration & dosage
Cytokines
/ metabolism
Dogs
Drug Design
Epithelial Cells
/ drug effects
Female
Fimbriae Proteins
/ genetics
Genotype
Gingiva
/ drug effects
Humans
Interferon-alpha
/ administration & dosage
Male
Periodontal Diseases
/ drug therapy
Porphyromonas
/ drug effects
Virulence
Virulence Factors
/ metabolism
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
20 02 2020
20 02 2020
Historique:
received:
16
04
2019
accepted:
29
01
2020
entrez:
22
2
2020
pubmed:
23
2
2020
medline:
31
12
2020
Statut:
epublish
Résumé
Porphyromonas gulae is a major periodontal pathogen in dogs, which can be transmitted to their owners. A major virulence factor of P. gulae consists of a 41-kDa filamentous appendage (FimA) on the cell surface, which is classified into three genotypes: A, B, and C. Thus far, inhibition of periodontal disease in dogs remains difficult. The present study assessed the inhibitory effects of a combination of clindamycin and interferon alpha (IFN-α) formulation against P. gulae and periodontal disease. Growth of P. gulae was significantly inhibited by clindamycin; this inhibition had a greater effect on type C P. gulae than on type A and B isolates. In contrast, the IFN-α formulation inhibited the expression of IL-1β and COX-2 elicited by type A and B isolates, but not that elicited by type C isolates. Furthermore, periodontal recovery was promoted by the administration of both clindamycin and IFN-α formulation to dogs undergoing periodontal treatment; moreover, this combined treatment reduced the number of FimA genotypes in oral specimens from treated dogs. These results suggest that a combination of clindamycin and IFN-α formulation inhibit P. gulae virulence and thus may be effective for the prevention of periodontal disease induced by P. gulae.
Identifiants
pubmed: 32080231
doi: 10.1038/s41598-020-59730-9
pii: 10.1038/s41598-020-59730-9
pmc: PMC7033253
doi:
Substances chimiques
Cytokines
0
Interferon-alpha
0
Virulence Factors
0
fimbrillin
0
Fimbriae Proteins
147680-16-8
Clindamycin
3U02EL437C
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3113Commentaires et corrections
Type : ErratumIn
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