Designing chimeric enzymes inspired by fungal cellulosomes.
Anaerobic fungi
BSA, Bovine serum albumin
CMC, Carboxymethyl cellulose
Cellulosome
DDP, Dockerin domain protein
DNS, Dinitrosalicylate
Dockerin
Enzyme
GH, Glycoside hydrolase
IPTG, Isopropyl-β- d-thiogalactopyranoside
Scaffoldin
Thermophile
pNPG, p-Nitrophenyl β-d-glucopyranoside
Journal
Synthetic and systems biotechnology
ISSN: 2405-805X
Titre abrégé: Synth Syst Biotechnol
Pays: China
ID NLM: 101694371
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
02
12
2019
revised:
11
01
2020
accepted:
13
01
2020
entrez:
22
2
2020
pubmed:
23
2
2020
medline:
23
2
2020
Statut:
epublish
Résumé
Cellulosomes are synthesized by anaerobic bacteria and fungi to degrade lignocellulose via synergistic action of multiple enzymes fused to a protein scaffold. Through templating key protein domains (cohesin and dockerin), designer cellulosomes have been engineered from bacterial motifs to alter the activity, stability, and degradation efficiency of enzyme complexes. Recently a parts list for fungal cellulosomes from the anaerobic fungi (
Identifiants
pubmed: 32083193
doi: 10.1016/j.synbio.2020.01.003
pii: S2405-805X(20)30003-X
pmc: PMC7015840
doi:
Types de publication
Journal Article
Langues
eng
Pagination
23-32Informations de copyright
© 2020 Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.
Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.
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