Non-typhoidal Salmonella intestinal carriage in a Schistosoma mansoni endemic community in a rural area of the Democratic Republic of Congo.
Adolescent
Adult
Animals
Carrier State
/ epidemiology
Child
Coinfection
/ epidemiology
Cross-Sectional Studies
Democratic Republic of the Congo
/ epidemiology
Female
Humans
Intestines
/ microbiology
Male
Rural Population
Salmonella typhimurium
/ genetics
Schistosoma mansoni
/ genetics
Schistosomiasis mansoni
/ epidemiology
Young Adult
Journal
PLoS neglected tropical diseases
ISSN: 1935-2735
Titre abrégé: PLoS Negl Trop Dis
Pays: United States
ID NLM: 101291488
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
30
05
2019
accepted:
25
10
2019
entrez:
22
2
2020
pubmed:
23
2
2020
medline:
25
4
2020
Statut:
epublish
Résumé
Clinical observations and animal studies have suggested that Salmonella intestinal carriage is promoted by concurrent Schistosoma infection. The present study assessed association of Salmonella intestinal carriage and Schistosoma mansoni infection among individuals in a Schistosoma endemic area in sub-Saharan Africa. From November 2015 to March 2016, a cross-sectional community-wide study was conducted in Kifua II, a rural village in Kongo Central Province, Democratic Republic of Congo. Stool samples were collected and analyzed for Salmonella intestinal carriage (culture) and Schistosoma mansoni infection (Kato Katz microscopy with determination of egg load). Salmonella Typhimurium and Enteritidis isolates were assessed for genetic similarity with blood culture isolates obtained during the same period in a neighboring hospital using multi-locus variable-numbers tandem repeat analysis (MLVA). A total of 1,108 participants were included (median age 15 years (IQR: 7-36), male-to-female ratio of 1:1.1). The overall prevalence of Schistosoma mansoni infection and non-typhoidal Salmonella carriage was 51.2% (95% CI: 48.2-54.1) and 3.4% (95% CI: 2.5-4.7) respectively, with 2.2% (95% CI: 1.5-3.2) of participants coinfected. The proportion of Salmonella carriage tended to be higher among Schistosoma mansoni infected participants compared to non-infected participants but this difference did not reach statistical significance (4.2% versus 2.6%, p = 0.132). However, the proportion of Salmonella carriage among participants with a heavy Schistosoma mansoni infection was significantly higher compared to those with a light and moderate infection (8.7% versus 3.2%, p = 0.012) and compared to Schistosoma mansoni negatives (8.7% versus 2.6%, p = 0.002). The 38 Salmonella isolates comprised five and four Enteritidis and Typhimurium serotypes respectively, the majority of them had MLVA types identical or similar to those observed among blood culture isolates. Salmonella intestinal carriage was associated with a heavy intensity of Schistosoma mansoni infection. Further studies are needed to address causation.
Sections du résumé
BACKGROUND
Clinical observations and animal studies have suggested that Salmonella intestinal carriage is promoted by concurrent Schistosoma infection. The present study assessed association of Salmonella intestinal carriage and Schistosoma mansoni infection among individuals in a Schistosoma endemic area in sub-Saharan Africa.
METHODS
From November 2015 to March 2016, a cross-sectional community-wide study was conducted in Kifua II, a rural village in Kongo Central Province, Democratic Republic of Congo. Stool samples were collected and analyzed for Salmonella intestinal carriage (culture) and Schistosoma mansoni infection (Kato Katz microscopy with determination of egg load). Salmonella Typhimurium and Enteritidis isolates were assessed for genetic similarity with blood culture isolates obtained during the same period in a neighboring hospital using multi-locus variable-numbers tandem repeat analysis (MLVA).
RESULTS
A total of 1,108 participants were included (median age 15 years (IQR: 7-36), male-to-female ratio of 1:1.1). The overall prevalence of Schistosoma mansoni infection and non-typhoidal Salmonella carriage was 51.2% (95% CI: 48.2-54.1) and 3.4% (95% CI: 2.5-4.7) respectively, with 2.2% (95% CI: 1.5-3.2) of participants coinfected. The proportion of Salmonella carriage tended to be higher among Schistosoma mansoni infected participants compared to non-infected participants but this difference did not reach statistical significance (4.2% versus 2.6%, p = 0.132). However, the proportion of Salmonella carriage among participants with a heavy Schistosoma mansoni infection was significantly higher compared to those with a light and moderate infection (8.7% versus 3.2%, p = 0.012) and compared to Schistosoma mansoni negatives (8.7% versus 2.6%, p = 0.002). The 38 Salmonella isolates comprised five and four Enteritidis and Typhimurium serotypes respectively, the majority of them had MLVA types identical or similar to those observed among blood culture isolates.
CONCLUSION
Salmonella intestinal carriage was associated with a heavy intensity of Schistosoma mansoni infection. Further studies are needed to address causation.
Identifiants
pubmed: 32084128
doi: 10.1371/journal.pntd.0007875
pii: PNTD-D-19-00791
pmc: PMC7034803
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0007875Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Clin Infect Dis. 2015 Nov 1;61 Suppl 4:S346-53
pubmed: 26449951
Clin Infect Dis. 2004 Jun 1;38(11):1644-5
pubmed: 15156460
Rev Infect Dis. 1984 May-Jun;6(3):345-56
pubmed: 6377442
Emerg Infect Dis. 2015 Jun;21(6):
pubmed: 25860298
Ann Trop Paediatr. 1993;13(1):45-53
pubmed: 7681645
Clin Infect Dis. 2016 Mar 15;62 Suppl 1:S50-5
pubmed: 26933022
Lancet Glob Health. 2017 Mar;5(3):e310-e323
pubmed: 28193398
PLoS Negl Trop Dis. 2016 Jun 21;10(6):e0004778
pubmed: 27326453
Clin Infect Dis. 1999 Nov;29(5):1151-6
pubmed: 10524956
PLoS Negl Trop Dis. 2012;6(11):e1921
pubmed: 23166855
Am J Trop Med Hyg. 1992 Feb;46(2):132-6
pubmed: 1539746
Rev Inst Med Trop Sao Paulo. 1971 Nov-Dec;13(6):399-404
pubmed: 5124649
Trop Med Health. 2011 Mar;39(1):9-15
pubmed: 22028607
Clin Microbiol Rev. 2015 Oct;28(4):939-67
pubmed: 26224883
Am J Trop Med Hyg. 1982 Mar;31(2):328-34
pubmed: 6803604
Parasit Vectors. 2011 Jun 28;4:123
pubmed: 21711539
Infect Immun. 1984 May;44(2):274-81
pubmed: 6143728
Parasit Vectors. 2015 Apr 22;8:241
pubmed: 25896512
PLoS One. 2015 Feb 18;10(2):e0117950
pubmed: 25693200
Parasit Vectors. 2015 Nov 19;8:601
pubmed: 26586232
J Clin Microbiol. 2003 Apr;41(4):1469-79
pubmed: 12682132
Lancet. 2012 Jun 30;379(9835):2489-2499
pubmed: 22587967
PLoS Pathog. 2016 Dec 1;12(12):e1005928
pubmed: 27907208
West Afr J Med. 1996 Jan-Mar;15(1):24-30
pubmed: 8652437
BMC Res Notes. 2015 Mar 03;8:64
pubmed: 25889776
Clin Infect Dis. 1994 Jan;18(1):103-5
pubmed: 8054417
Front Microbiol. 2014 Aug 04;5:391
pubmed: 25136336
J Infect Dis. 1985 Jun;151(6):1166-7
pubmed: 3998513
J Infect Dis. 1980 Feb;141(2):177-85
pubmed: 6988520
Adv Exp Med Biol. 2013;764:13-26
pubmed: 23654054
J Infect Dis. 1985 Oct;152(4):722-6
pubmed: 2864378
J Infect Dis. 1986 Jul;154(1):179-82
pubmed: 3086463
Am J Trop Med Hyg. 1984 Nov;33(6):1166-9
pubmed: 6439063
Am J Trop Med Hyg. 1967 Jul;16(4):462-72
pubmed: 5006466
Ann Clin Microbiol Antimicrob. 2016 May 17;15(1):32
pubmed: 27188991
Clin Infect Dis. 2003 Dec 15;37(12):e177-9
pubmed: 14689364
J Bacteriol. 2014 Aug 15;196(16):3036-44
pubmed: 24957617
Euro Surveill. 2011 Aug 11;16(32):
pubmed: 21871223
PLoS Negl Trop Dis. 2014 Dec 18;8(12):e3387
pubmed: 25521351
J Infect Dis. 1972 Mar;125(3):249-56
pubmed: 4622710
Emerg Infect Dis. 2012 Oct;18(10):1692-4
pubmed: 23017665
Clin Microbiol Rev. 2015 Oct;28(4):901-37
pubmed: 26180063