TACAN Is an Ion Channel Involved in Sensing Mechanical Pain.
TACAN
bilayer
ion channel
mechanosensitive
mechanotransduction
nociceptor
pain
patch clamp
pillar
Journal
Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066
Informations de publication
Date de publication:
05 03 2020
05 03 2020
Historique:
received:
22
07
2019
revised:
08
11
2019
accepted:
29
01
2020
pubmed:
23
2
2020
medline:
18
8
2020
entrez:
22
2
2020
Statut:
ppublish
Résumé
Mechanotransduction, the conversion of mechanical stimuli into electrical signals, is a fundamental process underlying essential physiological functions such as touch and pain sensing, hearing, and proprioception. Although the mechanisms for some of these functions have been identified, the molecules essential to the sense of pain have remained elusive. Here we report identification of TACAN (Tmem120A), an ion channel involved in sensing mechanical pain. TACAN is expressed in a subset of nociceptors, and its heterologous expression increases mechanically evoked currents in cell lines. Purification and reconstitution of TACAN in synthetic lipids generates a functional ion channel. Finally, a nociceptor-specific inducible knockout of TACAN decreases the mechanosensitivity of nociceptors and reduces behavioral responses to painful mechanical stimuli but not to thermal or touch stimuli. We propose that TACAN is an ion channel that contributes to sensing mechanical pain.
Identifiants
pubmed: 32084332
pii: S0092-8674(20)30114-8
doi: 10.1016/j.cell.2020.01.033
pii:
doi:
Substances chimiques
Ion Channels
0
Lipids
0
Tmem120A protein, mouse
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
956-967.e17Subventions
Organisme : CIHR
ID : MOP-136903
Pays : Canada
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2020. Published by Elsevier Inc.
Déclaration de conflit d'intérêts
Declaration of Interests R.S.-N. has a patent (US9551718B2) related to this work.