Dissociation of Adaptive Thermogenesis from Glucose Homeostasis in Microbiome-Deficient Mice.
adaptive thermogenesis
brown adipose tissue
browning
cold stress
commensal-depleted
energy expenditure
germ-free
gluconeogenesis
gut microbiome
high-fat diet
Journal
Cell metabolism
ISSN: 1932-7420
Titre abrégé: Cell Metab
Pays: United States
ID NLM: 101233170
Informations de publication
Date de publication:
03 03 2020
03 03 2020
Historique:
received:
19
04
2019
revised:
18
11
2019
accepted:
24
01
2020
pubmed:
23
2
2020
medline:
2
7
2021
entrez:
22
2
2020
Statut:
ppublish
Résumé
Recent studies suggest that a key mechanism whereby the gut microbiome influences energy balance and glucose homeostasis is through the recruitment of brown and beige adipocytes, primary mediators of the adaptive thermogenic response. To test this, we assessed energy expenditure and glucose metabolism in two complementary mouse models of gut microbial deficiency, which were exposed to a broad range of thermal and dietary stresses. Neither ablation of the gut microbiome, nor the substantial microbial perturbations induced by cold ambient temperatures, influenced energy expenditure during cold exposure or high-fat feeding. Nevertheless, we demonstrated a critical role for gut microbial metabolism in maintaining euglycemia through the production of amino acid metabolites that optimized hepatic TCA (tricarboxylic acid) cycle fluxes in support of gluconeogenesis. These results distinguish the dispensability of the gut microbiome for the regulation of energy expenditure from its critical contribution to the maintenance of glucose homeostasis.
Identifiants
pubmed: 32084379
pii: S1550-4131(20)30013-9
doi: 10.1016/j.cmet.2020.01.012
pmc: PMC7888548
mid: NIHMS1569003
pii:
doi:
Substances chimiques
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
592-604.e9Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK107717
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK056626
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK103046
Pays : United States
Organisme : NIDDK NIH HHS
ID : T32 DK007533
Pays : United States
Organisme : NIH HHS
ID : S10 OD020100
Pays : United States
Organisme : NIDDK NIH HHS
ID : R37 DK048873
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK034854
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
Références
Nature. 1979 Sep 6;281(5726):31-5
pubmed: 551265
Cell Metab. 2018 Oct 2;28(4):656-666.e1
pubmed: 30017358
Bioinformatics. 2010 Oct 1;26(19):2460-1
pubmed: 20709691
mSystems. 2015 Dec 22;1(1):
pubmed: 27822518
Mol Syst Biol. 2015 Oct 16;11(10):834
pubmed: 26475342
Nat Rev Endocrinol. 2014 Jan;10(1):24-36
pubmed: 24146030
Nat Methods. 2011 Dec 28;9(1):57-63
pubmed: 22205519
Infect Immun. 2008 Mar;76(3):907-15
pubmed: 18160481
Science. 2013 Sep 6;341(6150):1241214
pubmed: 24009397
Hepatology. 2019 Aug;70(2):496-510
pubmed: 30516845
Nat Med. 2015 Dec;21(12):1497-1501
pubmed: 26569380
Cell Host Microbe. 2018 Apr 11;23(4):458-469.e5
pubmed: 29649441
Mol Metab. 2016 Aug 26;5(10):948-958
pubmed: 27689007
Nature. 2012 Jun 13;486(7402):207-14
pubmed: 22699609
J Lipid Res. 2016 Jun;57(6):943-54
pubmed: 27080715
Mol Metab. 2017 Nov;6(11):1371-1380
pubmed: 29107285
Proc Natl Acad Sci U S A. 2018 Jan 23;115(4):E743-E752
pubmed: 29311302
Diabetes. 2014 Dec;63(12):4089-99
pubmed: 25056438
Cell Host Microbe. 2017 Nov 8;22(5):589-599
pubmed: 29120742
J Am Soc Nephrol. 2019 May;30(5):751-766
pubmed: 30940651
Gastroenterology. 2012 Oct;143(4):913-6.e7
pubmed: 22728514
Nutrients. 2019 Jun 16;11(6):
pubmed: 31208147
Diabetes. 2015 Jul;64(7):2352-60
pubmed: 26050667
Cell Metab. 2018 Jan 9;27(1):252-262.e3
pubmed: 29320705
Cell Metab. 2018 Dec 4;28(6):907-921.e7
pubmed: 30174308
JPEN J Parenter Enteral Nutr. 1997 Nov-Dec;21(6):357-65
pubmed: 9406136
Immunity. 2007 Apr;26(4):461-75
pubmed: 17398123
JCI Insight. 2017 Apr 20;2(8):
pubmed: 28422755
Gut Microbes. 2018;9(3):218-235
pubmed: 29227180
J Clin Invest. 2016 Dec 1;126(12):4430-4443
pubmed: 27775551
Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15718-23
pubmed: 15505215
PLoS One. 2012;7(6):e39748
pubmed: 22768116
Nat Med. 2017 Dec;23(12):1454-1465
pubmed: 29131158
Nature. 2012 Jun 13;486(7402):215-21
pubmed: 22699610
Cell Rep. 2019 Mar 5;26(10):2720-2737.e5
pubmed: 30840893
J Exp Med. 1968 Jul 1;128(1):97-110
pubmed: 5662019
ISME J. 2012 Aug;6(8):1621-4
pubmed: 22402401
Nature. 2016 Dec 22;540(7634):544-551
pubmed: 27906159
Cell Metab. 2017 Nov 7;26(5):801
pubmed: 29117546
Am J Physiol Gastrointest Liver Physiol. 2013 Dec;305(12):G900-10
pubmed: 24136789
Science. 2013 Aug 2;341(6145):569-73
pubmed: 23828891
Int J Obes Relat Metab Disord. 1999 Nov;23(11):1105-17
pubmed: 10578199
Cell. 2014 Jan 16;156(1-2):84-96
pubmed: 24412651
Nat Methods. 2013 Oct;10(10):996-8
pubmed: 23955772
Compr Physiol. 2014 Jan;4(1):177-97
pubmed: 24692138
Mol Metab. 2016 Jul 18;5(9):795-803
pubmed: 27617202
J Endocrinol Invest. 2008 Aug;31(8):694-9
pubmed: 18852529
Nutr Cancer. 1997;28(2):212-7
pubmed: 9290130
Am J Physiol. 1990 Feb;258(2 Pt 2):R552-7
pubmed: 2309941
Am J Physiol Endocrinol Metab. 2017 Nov 1;313(5):E515-E527
pubmed: 28679625
Mol Metab. 2016 Oct 13;5(12):1162-1174
pubmed: 27900259
Cell Metab. 2015 Oct 6;22(4):546-59
pubmed: 26445512
Nucleic Acids Res. 2013 Jan;41(Database issue):D590-6
pubmed: 23193283
Front Physiol. 2013 Mar 14;4:34
pubmed: 23504620
J Biol Chem. 2006 Oct 20;281(42):31894-908
pubmed: 16914547
J Histochem Cytochem. 2014 Apr;62(4):237-50
pubmed: 24453191
Nat Med. 2017 Jul;23(7):839-849
pubmed: 28604703
Physiol Rev. 2004 Jan;84(1):277-359
pubmed: 14715917
Exp Mol Med. 2012 Nov 30;44(11):665-73
pubmed: 23114424
Mol Metab. 2016 Feb 23;5(5):340-351
pubmed: 27110486
Cell Metab. 2016 Jun 14;23(6):1216-1223
pubmed: 27304513
Mol Cell. 2017 Jun 15;66(6):789-800
pubmed: 28622524
Nat Methods. 2017 Apr 27;14(5):461-462
pubmed: 28448069
PLoS One. 2010 May 28;5(5):e10883
pubmed: 20526369
Cell. 2015 Dec 3;163(6):1360-74
pubmed: 26638070
Cell Metab. 2018 Aug 7;28(2):207-216.e3
pubmed: 29909972
Proc Natl Acad Sci U S A. 2019 Feb 19;116(8):3171-3176
pubmed: 30718429
Diabetes. 2015 Jul;64(7):2369-75
pubmed: 26050669
Nat Protoc. 2012 Mar 01;7(3):606-15
pubmed: 22383039
J Physiol. 1949 Aug;109(1-2):1-9
pubmed: 15394301
Anal Chem. 2012 Jun 5;84(11):5035-9
pubmed: 22533540
Endocrinology. 2008 Jul;149(7):3478-89
pubmed: 18372322
Biochimie. 2009 Feb;91(2):261-70
pubmed: 18957317
Cell Metab. 2014 Nov 4;20(5):779-786
pubmed: 25156449
Nucleic Acids Res. 2018 Jul 2;46(W1):W486-W494
pubmed: 29762782
Nucleic Acids Res. 2014 Jan;42(Database issue):D633-42
pubmed: 24288368
Br J Nutr. 2012 Mar;107(5):621-30
pubmed: 21781379
J Nutr. 2011 Apr 1;141(4):582-7
pubmed: 21310864
Nephrology (Carlton). 2018 Oct;23 Suppl 4:16-20
pubmed: 30298666
Cell. 2015 Oct 22;163(3):643-55
pubmed: 26496606
Genome Biol. 2011 Jun 24;12(6):R60
pubmed: 21702898
Cell Metab. 2017 Oct 3;26(4):693
pubmed: 28978428
Nutrients. 2015 Apr 16;7(4):2930-46
pubmed: 25894657
J Microbiol Biotechnol. 2015 Oct;25(10):1583-8
pubmed: 26032361
J Int Soc Sports Nutr. 2014 Feb 24;11(1):5
pubmed: 24565110
Cell Host Microbe. 2018 Apr 11;23(4):447-457.e4
pubmed: 29576480
Cell. 2018 Nov 29;175(6):1561-1574.e12
pubmed: 30449620
Hepatology. 2018 Jan;67(1):145-158
pubmed: 28802074
J Biol Chem. 2013 Nov 15;288(46):33376-86
pubmed: 24072708
Nat Rev Immunol. 2009 May;9(5):313-23
pubmed: 19343057
J Clin Microbiol. 1997 Jun;35(6):1353-60
pubmed: 9163443
PLoS One. 2018 Oct 25;13(10):e0198305
pubmed: 30359379
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6554-9
pubmed: 23576728