RNA-Guided Genomic Localization of H2A.L.2 Histone Variant.
centromere
epigenetic inheritance
histone dynamics
repetitive elements
testis-specific histone variants
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
18 02 2020
18 02 2020
Historique:
received:
31
01
2020
revised:
14
02
2020
accepted:
16
02
2020
entrez:
23
2
2020
pubmed:
23
2
2020
medline:
20
2
2021
Statut:
epublish
Résumé
The molecular basis of residual histone retention after the nearly genome-wide histone-to-protamine replacement during late spermatogenesis is a critical and open question. Our previous investigations showed that in postmeiotic male germ cells, the genome-scale incorporation of histone variants TH2B-H2A.L.2 allows a controlled replacement of histones by protamines to occur. Here, we highlight the intrinsic ability of H2A.L.2 to specifically target the pericentric regions of the genome and discuss why pericentric heterochromatin is a privileged site of histone retention in mature spermatozoa. We observed that the intranuclear localization of H2A.L.2 is controlled by its ability to bind RNA, as well as by an interplay between its RNA-binding activity and its tropism for pericentric heterochromatin. We identify the H2A.L.2 RNA-binding domain and demonstrate that in somatic cells, the replacement of H2A.L.2 RNA-binding motif enhances and stabilizes its pericentric localization, while the forced expression of RNA increases its homogenous nuclear distribution. Based on these data, we propose that the specific accumulation of RNA on pericentric regions combined with H2A.L.2 tropism for these regions are responsible for stabilizing H2A.L.2 on these regions in mature spermatozoa. This situation would favor histone retention on pericentric heterochromatin.
Identifiants
pubmed: 32085641
pii: cells9020474
doi: 10.3390/cells9020474
pmc: PMC7072763
pii:
doi:
Substances chimiques
Heterochromatin
0
Histones
0
RNA Recognition Motif Proteins
0
RNA, Nuclear
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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