Cadmium elicits alterations in mitochondrial morphology and functionality in C3H10T1/2Cl8 mouse embryonic fibroblasts.
Cadmium
Carcinogenesis
Cell metabolism
DNA damage
Mitochondria
Reactive oxygen species
Journal
Biochimica et biophysica acta. General subjects
ISSN: 1872-8006
Titre abrégé: Biochim Biophys Acta Gen Subj
Pays: Netherlands
ID NLM: 101731726
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
29
10
2019
revised:
28
01
2020
accepted:
18
02
2020
pubmed:
23
2
2020
medline:
22
12
2020
entrez:
23
2
2020
Statut:
ppublish
Résumé
Cadmium is a widespread carcinogen. We previously showed that the administration of low CdCl Respiratory metabolism was investigated through Seahorse Agilent assays, while oxidative stress level was assessed through fluorescent probes; DNA damage was evaluated by Comet assay, and mitochondrial morphology was analyzed in confocal microscopy. Results show that the initial response to CdCl Our results confirm previously shown response against cadmium toxicity; new data about glycolytic increase and mitochondrial rearrangements suggest pathways leading to cell transformation. In this work we exploit the widely used, well known CTA, which allows following healthy cells transformation into a malignant phenotype, to understand early events in cadmium-induced carcinogenesis.
Sections du résumé
BACKGROUND
Cadmium is a widespread carcinogen. We previously showed that the administration of low CdCl
METHODS
Respiratory metabolism was investigated through Seahorse Agilent assays, while oxidative stress level was assessed through fluorescent probes; DNA damage was evaluated by Comet assay, and mitochondrial morphology was analyzed in confocal microscopy.
RESULTS
Results show that the initial response to CdCl
CONCLUSIONS
Our results confirm previously shown response against cadmium toxicity; new data about glycolytic increase and mitochondrial rearrangements suggest pathways leading to cell transformation.
GENERAL SIGNIFICANCE
In this work we exploit the widely used, well known CTA, which allows following healthy cells transformation into a malignant phenotype, to understand early events in cadmium-induced carcinogenesis.
Identifiants
pubmed: 32087270
pii: S0304-4165(20)30058-1
doi: 10.1016/j.bbagen.2020.129568
pii:
doi:
Substances chimiques
Cadmium Chloride
J6K4F9V3BA
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
129568Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.