In Vitro Cellular Delivery of Peptide Nucleic Acid (PNA).
CPP
Cell-penetrating peptides
PNA conjugates
Photochemical internalization (PCI)
Transfection
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
24
2
2020
pubmed:
24
2
2020
medline:
20
1
2021
Statut:
ppublish
Résumé
Cellular delivery methods are a prerequisite for cellular studies with PNA. This chapter describes PNA cellular delivery using cell-penetrating peptide (CPP)-PNA conjugates and transfection of PNA-ligand conjugates mediated by cationic lipids. Furthermore, two endosomolytic procedures employing chloroquine treatment or photochemical internalization (PCI) for significantly improving PNA delivery efficacy are described.
Identifiants
pubmed: 32088870
doi: 10.1007/978-1-0716-0243-0_10
doi:
Substances chimiques
Lipids
0
Peptide Nucleic Acids
0
Cholic Acid
G1JO7801AE
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM