Detection of Microorganisms by Fluorescence In Situ Hybridization Using Peptide Nucleic Acid.
Biofilms
Enriched samples
FISH
Histological samples
Microorganisms
PNA
Pure cultures
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
24
2
2020
pubmed:
24
2
2020
medline:
20
1
2021
Statut:
ppublish
Résumé
Fluorescence in situ hybridization (FISH) is a 30-year-old technology that has evolved continuously and is now one of the most well-established molecular biology techniques. Traditionally, DNA probes are used for in situ hybridization. However, synthetic molecules are emerging as very promising alternatives, providing better hybridization performance and making FISH procedures easier and more efficient. In this chapter, we describe a universal FISH protocol, using nucleic acid probes, for the detection of bacteria. This protocol should be easily applied to different microorganisms as a way of identifying in situ relevant microorganisms (including pathogens) and their distribution patterns in different types of samples.
Identifiants
pubmed: 32088873
doi: 10.1007/978-1-0716-0243-0_13
doi:
Substances chimiques
Nucleic Acid Probes
0
Peptide Nucleic Acids
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM