Mycobacterium tuberculosis Complex Lineage 3 as Causative Agent of Pulmonary Tuberculosis, Eastern Sudan
Adult
Antitubercular Agents
/ pharmacology
Bacterial Typing Techniques
Drug Resistance, Multiple, Bacterial
/ genetics
Female
Humans
Male
Microbial Sensitivity Tests
Middle Aged
Mycobacterium tuberculosis
/ drug effects
Sputum
/ microbiology
Sudan
/ epidemiology
Tuberculosis, Pulmonary
/ epidemiology
MDR
MDR TB
Mycobacterium tuberculosis complex
Sudan
antimicrobial resistance
bacteria
lineage 3
transmission
tuberculosis and other mycobacteria
whole-genome sequencing
Journal
Emerging infectious diseases
ISSN: 1080-6059
Titre abrégé: Emerg Infect Dis
Pays: United States
ID NLM: 9508155
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
entrez:
25
2
2020
pubmed:
25
2
2020
medline:
16
3
2021
Statut:
ppublish
Résumé
Pathogen-based factors associated with tuberculosis (TB) in eastern Sudan are not well defined. We investigated genetic diversity, drug resistance, and possible transmission clusters of Mycobacterium tuberculosis complex (MTBC) strains by using a genomic epidemiology approach. We collected 383 sputum specimens at 3 hospitals in 2014 and 2016 from patients with symptoms suggestive of TB; of these, 171 grew MTBC strains. Whole-genome sequencing could be performed on 166 MTBC strains; phylogenetic classification revealed that most (73.4%; n = 122) belonged to lineage 3 (L3). Genome-based cluster analysis showed that 76 strains (45.9%) were grouped into 29 molecular clusters, comprising 2-8 strains/patients. Of the strains investigated, 9.0% (15/166) were multidrug resistant (MDR); 10 MDR MTBC strains were linked to 1 large MDR transmission network. Our findings indicate that L3 strains are the main causative agent of TB in eastern Sudan; MDR TB is caused mainly by transmission of MDR L3 strains.
Identifiants
pubmed: 32091355
doi: 10.3201/eid2603.191145
pmc: PMC7045825
doi:
Substances chimiques
Antitubercular Agents
0
Types de publication
Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
427-436Références
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