Salt gradient chromatographic separation of chondroitin sulfate disaccharides.
Capillary liquid chromatography
Chondroitin sulfate
Glycosaminoglycan
Liver cancer
Salt gradient
Journal
Journal of chromatography. A
ISSN: 1873-3778
Titre abrégé: J Chromatogr A
Pays: Netherlands
ID NLM: 9318488
Informations de publication
Date de publication:
24 May 2020
24 May 2020
Historique:
received:
10
11
2019
revised:
13
02
2020
accepted:
13
02
2020
pubmed:
26
2
2020
medline:
7
7
2020
entrez:
26
2
2020
Statut:
ppublish
Résumé
In the present study, we describe the development of a fast, 2-step salt gradient for analysis of chondroitin sulfate disaccharides. Using salt gradients, which is somewhat unusual in HILIC-based separations, provides relatively fast chromatography with excellent sensitivity (15 min cycle time, 10-20 fmol/µL detection, 30-50 fmol/µL quantitation limit), and good linearity. The efficiency of the new method is demonstrated by measuring human tissue slices of healthy, cirrhotic, and cancerous liver samples. Preliminary results show major differences among the quantity and sulfation pattern of the various sample types.
Identifiants
pubmed: 32093904
pii: S0021-9673(20)30178-3
doi: 10.1016/j.chroma.2020.460979
pii:
doi:
Substances chimiques
Disaccharides
0
Sodium Chloride
451W47IQ8X
Chondroitin Sulfates
9007-28-7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
460979Informations de copyright
Copyright © 2020. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.