The effect of hepatopancreas homogenate of the Red king crab on HA-based filler.
Atomic force microscopy
Hepatopancreas
Hyaluronic acid
Hyaluronidase
Hyaluronidase activity
Nuclear magnetic resonance
Red king crab
Treating filler complications
Turbidimetric method
Journal
PeerJ
ISSN: 2167-8359
Titre abrégé: PeerJ
Pays: United States
ID NLM: 101603425
Informations de publication
Date de publication:
2020
2020
Historique:
received:
22
11
2019
accepted:
16
01
2020
entrez:
26
2
2020
pubmed:
26
2
2020
medline:
26
2
2020
Statut:
epublish
Résumé
In this study, several methods were used to analyze the hydrolysis of hyaluronic acid (HA)-based cosmetic fillers by the hepatopancreas homogenate of the Red king crab. The results show that the homogenate and commercially available hyaluronidases have similar hydrolysis activities on the fillers. Atomic force microscopy images reveal that the HA fillers consist mainly of spherical-like particles, which are converted into filamentous structures as a result of hydrolysis by the Red king crab hepatopancreas homogenate. Turbidimetric analysis of the hydrolysis process shows that HA aggregation with acidic albumin exhibits a bell-shaped dependence on reaction time. Analysis of the hydrolysis process by nuclear magnetic resonance shows that HA degradation lasts several days. The maximum rate of the reaction is detected in the 1st h of incubation. The data confirm that the purified homogenate of the Red king crab hepatopancreas exerts hyaluronidase activity on HA-based cosmetic fillers; therefore, it may be considered as a potential therapeutic agent for treating filler complications.
Identifiants
pubmed: 32095375
doi: 10.7717/peerj.8579
pii: 8579
pmc: PMC7023832
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e8579Informations de copyright
© 2020 Ponomareva et al.
Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.
Références
Nature. 1970 Aug 15;227(5259):680-5
pubmed: 5432063
Am J Pathol. 1996 Jun;148(6):1721-6
pubmed: 8669457
Int J Biol Macromol. 2019 Jan;121:556-571
pubmed: 30321638
J Struct Biol. 1999 Jun 1;126(1):52-8
pubmed: 10329488
Bull Exp Biol Med. 2008 Jul;146(1):81-2
pubmed: 19145357
J Plast Reconstr Aesthet Surg. 2011 Jul;64(7):892-6
pubmed: 21310674
J Biol Chem. 1948 Feb;172(2):367-75
pubmed: 18901154
Biochem J. 1991 Mar 15;274 ( Pt 3):699-705
pubmed: 2012600
Biophys J. 1998 Oct;75(4):2030-7
pubmed: 9746545
J Biol Chem. 1948 Feb;172(2):511-4
pubmed: 18901169
J Biol Chem. 1950 Oct;186(2):615-23
pubmed: 14794656
Clin Exp Rheumatol. 1994 Jan-Feb;12(1):75-82
pubmed: 8162648
Int J Biol Macromol. 1990 Jun;12(3):180-4
pubmed: 2125466
Plast Reconstr Surg. 2013 Oct;132(4 Suppl 2):22S-32S
pubmed: 24077007