Diagnostic and follow-up performance of serological tests for different forms/courses of alveolar echinococcosis.
ABZ, Albendazole
AE, Alveolar echinococcosis
CE, Cystic echinococcosis
Diagnosis
ELISA
ELISA, Enzyme-linked immunosorbent assay
Echinococcus multilocularis
EgHF, Echinococcus granulosus hydatid fluid
Em, Echinococcus multilocularis
EmVF, Echinococcus multilocularis vesicular fluid
FDG-PET/CT, fluorodeoxyglucose Positron Emission Tomography/Computed Tomography
Follow-up serology
Immunoblotting
MRI, Magnetic Resonance Imaging
US, Ultrasonography
Journal
Food and waterborne parasitology
ISSN: 2405-6766
Titre abrégé: Food Waterborne Parasitol
Pays: Netherlands
ID NLM: 101762332
Informations de publication
Date de publication:
Sep 2019
Sep 2019
Historique:
received:
27
02
2019
revised:
15
04
2019
accepted:
16
04
2019
entrez:
26
2
2020
pubmed:
26
2
2020
medline:
26
2
2020
Statut:
epublish
Résumé
Diagnosis of alveolar echinococcosis (AE) is predominantly based on imaging procedures combined with immunodiagnostic testing. In the present study, we retrospectively analyzed the performance of four serological tests (EgHF-ELISA, Em2-ELISA, recEm18-ELISA and Em-Immunoblotting) for initial diagnosis and subsequent monitoring of AE patients. Overall, 101 AE patients were included, grouped according to treatment options and immune status as follows: (A) curative surgical treatment (n = 45 patients), (B) non-radical or palliative surgical treatment (n = 11), (C) benzimidazoles only (n = 20), (D) immunocompromised with radical surgical treatment (n = 11), (E) immunocompromised with benzimidazoles only (n = 4), and finally a group of 10 AE patients (F) that were considered to present so-called "abortive" lesions. Initial (i.e. pretreatment) ELISA-based diagnosis for patients in groups A to E revealed overall diagnostic sensitivities of 95% for EgHF, 86% for Em2, and 80% for recEm18, respectively. Comparatively, the diagnostic sensitivity of Em-Immunoblotting was higher with an overall value of 98%. In group F, only Em-Immunoblotting had an excellent diagnostic sensitivity (100%), whereas the ELISAs had poor sensitivities of 30% (EgHF- and Em2-ELISA) or even 0% (recEm18-ELISA). Serological monitoring of AE patients showed a clear association between a curative development of disease (induced either by surgery or benzimidazole medication) and a negativization in the ELISAs. This effect was most pronounced for the recEm18-ELISA, where 56% negativized following diagnosis/treatment, as compared to 36% for the EgHF-ELISA, and 37% for the Em2-ELISA, respectively. After radical surgery, the mean time until negativization in the recEm18-ELISA was 2.4 years (SD 1.6). This was significantly shorter than the mean 3.9 years (SD 2.5) in those AE patients with non-radical, palliative surgery or ABZ treatment who were able to negativize during the study period (
Identifiants
pubmed: 32095626
doi: 10.1016/j.fawpar.2019.e00055
pii: S2405-6766(19)30015-0
pii: e00055
pmc: PMC7034017
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e00055Informations de copyright
© 2019 The Authors.
Références
Am J Trop Med Hyg. 2009 May;80(5):792-7
pubmed: 19407126
J Helminthol. 2009 Mar;83(1):57-61
pubmed: 19025727
Chin Med J (Engl). 2011 Sep;124(18):2824-8
pubmed: 22167824
GMS Infect Dis. 2017 Jan 06;5:Doc01
pubmed: 30671323
Med Microbiol Immunol. 2014 Jun;203(3):185-93
pubmed: 24509604
PLoS Negl Trop Dis. 2015 Sep 21;9(9):e0003964
pubmed: 26389799
J Clin Microbiol. 2003 Jul;41(7):3351-3
pubmed: 12843091
Infection. 2004 Oct;32(5):299-302
pubmed: 15624896
Parasitology. 2007 Jun;134(Pt 6):911-20
pubmed: 17288631
Clin Microbiol Infect. 2010 Jun;16(6):788-91
pubmed: 19912267
J Clin Microbiol. 1993 Feb;31(2):373-6
pubmed: 8432825
Trends Parasitol. 2015 Sep;31(9):407-12
pubmed: 26115902
Clin Microbiol Rev. 2019 Feb 13;32(2):
pubmed: 30760475
Z Parasitenkd. 1983;69(6):737-48
pubmed: 6659651
J Clin Microbiol. 2002 Nov;40(11):4161-5
pubmed: 12409391
Exp Ther Med. 2018 Oct;16(4):3155-3160
pubmed: 30214538
Adv Parasitol. 2017;96:159-257
pubmed: 28212789
Hepatology. 1999 Oct;30(4):857-64
pubmed: 10498634
Am J Trop Med Hyg. 1994 Dec;51(6):837-46
pubmed: 7810820
Clin Vaccine Immunol. 2010 Mar;17(3):470-5
pubmed: 20042519
Z Parasitenkd. 1983;69(3):347-56
pubmed: 6192602
Expert Rev Anti Infect Ther. 2016 Dec;14(12):1179-1194
pubmed: 27686694
Parasite. 2014;21:63
pubmed: 25429386
J Pediatr. 1997 Feb;130(2):320-3
pubmed: 9042141
Am J Trop Med Hyg. 1987 May;36(3):576-85
pubmed: 3578654
Trop Med Int Health. 2001 Jun;6(6):463-75
pubmed: 11422961
Clin Vaccine Immunol. 2010 Jun;17(6):944-8
pubmed: 20392888
Vet Parasitol. 2015 Oct 30;213(3-4):103-9
pubmed: 26260407
Am J Trop Med Hyg. 1999 Feb;60(2):193-8
pubmed: 10072135
Transplantation. 2003 Mar 27;75(6):856-63
pubmed: 12660515
Parasitol Int. 2006;55 Suppl:S51-5
pubmed: 16360335
J Clin Microbiol. 2009 Oct;47(10):3191-6
pubmed: 19656973
Trends Parasitol. 2003 Sep;19(9):377-81
pubmed: 12957509
Acta Trop. 2010 Apr;114(1):1-16
pubmed: 19931502
Nuklearmedizin. 2015;54(1):43-9
pubmed: 25423884
Parasitology. 2007 Jun;134(Pt 6):879-88
pubmed: 17306060
Curr Opin Infect Dis. 2010 Oct;23(5):505-12
pubmed: 20683265
J Hepatol. 2004 Oct;41(4):551-9
pubmed: 15532108
Liver Transpl. 2011 Jul;17(7):855-65
pubmed: 21455928
Clin Lab. 2009;55(1-2):41-8
pubmed: 19350848
World J Surg. 2017 Apr;41(4):1012-1018
pubmed: 27822723