Nucleocytoplasmic transport of intrinsically disordered proteins studied by high-speed super-resolution microscopy.
intrinsically disordered proteins (IDPs)
nuclear pore complex (NPC)
nucleocytoplasmic transport
super-resolution
Journal
Protein science : a publication of the Protein Society
ISSN: 1469-896X
Titre abrégé: Protein Sci
Pays: United States
ID NLM: 9211750
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
01
11
2019
revised:
18
02
2020
accepted:
18
02
2020
pubmed:
26
2
2020
medline:
24
2
2021
entrez:
26
2
2020
Statut:
ppublish
Résumé
Both natively folded and intrinsically disordered proteins (IDPs) destined for the nucleus need to transport through the nuclear pore complexes (NPCs) in eukaryotic cells. NPCs allow for passive diffusion of small folded proteins while barricading large ones, unless they are facilitated by nuclear transport receptors. However, whether nucleocytoplasmic transport of IDPs would follow these rules remains unknown. By using a high-speed super-resolution fluorescence microscopy, we have measured transport kinetics and 3D spatial locations of transport routes through native NPCs for various IDPs. Our data revealed that the rules executed for folded proteins are not well followed by the IDPs. Instead, both large and small IDPs can passively diffuse through the NPCs. Furthermore, their diffusion efficiencies and routes are differentiated by their content ratio of charged (Ch) and hydrophobic (Hy) amino acids. A Ch/Hy-ratio mechanism was finally suggested for nucleocytoplasmic transport of IDPs.
Identifiants
pubmed: 32096308
doi: 10.1002/pro.3845
pmc: PMC7255516
doi:
Substances chimiques
Intrinsically Disordered Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1459-1472Subventions
Organisme : NIH HHS
ID : GM094041
Pays : United States
Organisme : NIGMS NIH HHS
ID : R15 GM094041
Pays : United States
Organisme : NIH HHS
ID : GM122552
Pays : United States
Organisme : NIH HHS
ID : GM116204
Pays : United States
Organisme : NIH HHS
ID : GM097037
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM097037
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM122552
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA009171
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM116204
Pays : United States
Informations de copyright
© 2020 The Protein Society.
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