Uterine Dnmt3a is not Required for Mouse Embryo Implantation.
Dnmt3a
conditional knockout
decidualization
embryo implantation
endometrial receptivity
uterus
Journal
Current molecular medicine
ISSN: 1875-5666
Titre abrégé: Curr Mol Med
Pays: Netherlands
ID NLM: 101093076
Informations de publication
Date de publication:
2020
2020
Historique:
received:
18
07
2019
revised:
11
02
2020
accepted:
11
02
2020
pubmed:
26
2
2020
medline:
23
11
2021
entrez:
26
2
2020
Statut:
ppublish
Résumé
Recent studies have demonstrated that endometrial DNA methylation is essential for embryo implantation during early pregnancy. Dnmt3a is one of the key enzymes for DNA methylation and could be expressed in the endometrium regularly at this stage. In this study, we conditionally ablated uterine Dnmt3a using progesterone receptor-cre (Pgrcre) to define the physiological roles of Dnmt3a in female reproduction. We found that ovarian function was not apparently altered and the number of embryo implantation sites in Dnmt3a Overall, these results demonstrated that although some decidual markers are expressed abnormally, conditional knockout of Dnmt3a in the uterus did not significantly affect the endometrial function during embryo implantation; the embryo could implant into the endometrium normally.
Sections du résumé
BACKGROUND
Recent studies have demonstrated that endometrial DNA methylation is essential for embryo implantation during early pregnancy. Dnmt3a is one of the key enzymes for DNA methylation and could be expressed in the endometrium regularly at this stage.
OBJECTIVE AND METHODS
In this study, we conditionally ablated uterine Dnmt3a using progesterone receptor-cre (Pgrcre) to define the physiological roles of Dnmt3a in female reproduction.
RESULTS
We found that ovarian function was not apparently altered and the number of embryo implantation sites in Dnmt3a
CONCLUSION
Overall, these results demonstrated that although some decidual markers are expressed abnormally, conditional knockout of Dnmt3a in the uterus did not significantly affect the endometrial function during embryo implantation; the embryo could implant into the endometrium normally.
Identifiants
pubmed: 32096741
pii: CMM-EPUB-104814
doi: 10.2174/1566524020666200225115526
doi:
Substances chimiques
Dnmt3a protein, mouse
0
DNA (Cytosine-5-)-Methyltransferases
EC 2.1.1.37
DNA Methyltransferase 3A
EC 2.1.1.37
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
633-642Subventions
Organisme : National Natural Science Foundation Project of China
ID : 31571190
Organisme : Natural Science Foundation of Chongqing
ID : CSTC2018JCYJAX0309
Organisme : Chongqing Graduate Student Research Innovation Project
ID : CYB16096, CYS16117
Informations de copyright
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