Fat Graft Enrichment Strategies: A Systematic Review.
Adipose Tissue
/ cytology
Animals
Autografts
/ physiology
Body Contouring
/ adverse effects
Esthetics
Graft Survival
/ physiology
Humans
Mesenchymal Stem Cell Transplantation
/ adverse effects
Models, Animal
Platelet-Rich Plasma
/ physiology
Transplantation, Autologous
/ adverse effects
Treatment Outcome
Journal
Plastic and reconstructive surgery
ISSN: 1529-4242
Titre abrégé: Plast Reconstr Surg
Pays: United States
ID NLM: 1306050
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
entrez:
26
2
2020
pubmed:
26
2
2020
medline:
20
6
2020
Statut:
ppublish
Résumé
Autologous fat grafting is a dynamic modality used in plastic surgery as an adjunct to improve functional and aesthetic form. However, current practices in fat grafting for soft-tissue augmentation are plagued by tremendous variability in long-term graft retention, resulting in suboptimal outcomes and repetitive procedures. This systematic review identifies and critically appraises the evidence for various enrichment strategies that can be used to augment and improve the viability of fat grafts. A comprehensive literature search of the Medline and PubMed databases was conducted for animal and human studies published through October of 2017 with multiple search terms related to adipose graft enrichment agents encompassing growth factors, platelet-rich plasma, adipose-derived and bone marrow stem cells, gene therapy, tissue engineering, and other strategies. Data on level of evidence, techniques, complications, and outcomes were collected. A total of 1382 articles were identified, of which 147 met inclusion criteria. The majority of enrichment strategies demonstrated positive benefit for fat graft survival, particularly with growth factors and adipose-derived stem cell enrichment. Platelet-rich plasma and adipose-derived stem cells had the strongest evidence to support efficacy in human studies and may demonstrate a dose-dependent effect. Improved understanding of enrichment strategies contributing to fat graft survival can help to optimize safety and outcomes. Controlled clinical studies are lacking, and future studies should examine factors influencing graft survival through controlled clinical trials in order to establish safety and to obtain consistent outcomes.
Sections du résumé
BACKGROUND
Autologous fat grafting is a dynamic modality used in plastic surgery as an adjunct to improve functional and aesthetic form. However, current practices in fat grafting for soft-tissue augmentation are plagued by tremendous variability in long-term graft retention, resulting in suboptimal outcomes and repetitive procedures. This systematic review identifies and critically appraises the evidence for various enrichment strategies that can be used to augment and improve the viability of fat grafts.
METHODS
A comprehensive literature search of the Medline and PubMed databases was conducted for animal and human studies published through October of 2017 with multiple search terms related to adipose graft enrichment agents encompassing growth factors, platelet-rich plasma, adipose-derived and bone marrow stem cells, gene therapy, tissue engineering, and other strategies. Data on level of evidence, techniques, complications, and outcomes were collected.
RESULTS
A total of 1382 articles were identified, of which 147 met inclusion criteria. The majority of enrichment strategies demonstrated positive benefit for fat graft survival, particularly with growth factors and adipose-derived stem cell enrichment. Platelet-rich plasma and adipose-derived stem cells had the strongest evidence to support efficacy in human studies and may demonstrate a dose-dependent effect.
CONCLUSIONS
Improved understanding of enrichment strategies contributing to fat graft survival can help to optimize safety and outcomes. Controlled clinical studies are lacking, and future studies should examine factors influencing graft survival through controlled clinical trials in order to establish safety and to obtain consistent outcomes.
Identifiants
pubmed: 32097333
doi: 10.1097/PRS.0000000000006557
pii: 00006534-202003000-00045
doi:
Types de publication
Journal Article
Systematic Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
827-841Commentaires et corrections
Type : CommentIn
Type : CommentIn
Références
Neuber F. Fetttransplantation. 22:66.
Khouri RK Jr.. Discussion: Enhancement of progenitor cells by two-step centrifugation of emulsified lipoaspirates. Plast Reconstr Surg. 2018;142:110–111.
Pallua N, Grasys J, Kim BS. Enhancement of progenitor cells by two-step centrifugation of emulsified lipoaspirates. Plast Reconstr Surg. 2018;142:99–109.
Canizares O Jr, Thomson JE, Allen RJ Jr, et al. The effect of processing technique on fat graft survival. Plast Reconstr Surg. 2017;140:933–943.
Vyas SaVH. Regulatory issues regarding fat grafting. Plastic Surgery Pulse News 2015:7.
Kato H, Mineda K, Eto H, et al. Degeneration, regeneration, and cicatrization after fat grafting: Dynamic total tissue remodeling during the first 3 months. Plast Reconstr Surg. 2014;133:303e–313e.
Pu LL. Mechanisms of fat graft survival. Ann Plast Surg. 2016;77(Suppl 1):S84—S86.
Pu LL, Yoshimura K, Coleman SR. Fat grafting: Current concept, clinical application, and regenerative potential. Part 1. Clin Plast Surg. 2015:42:ix–x.
Pu LL, Yoshimura K, Coleman SR. Fat grafting: Current concept, clinical application, and regenerative potential. Part 2. Preface. Clin Plast Surg. 2015;42:xiii–xxiv.
Small K, Choi M, Petruolo O, Lee C, Karp N. Is there an ideal donor site of fat for secondary breast reconstruction? Aesthet Surg J. 2014;34:545–550.
Choi M, Small K, Levovitz C, Lee C, Fadl A, Karp NS. The volumetric analysis of fat graft survival in breast reconstruction. Plast Reconstr Surg. 2013;131:185–191.
Lee JH, Kirkham JC, McCormack MC, Nicholls AM, Randolph MA, Austen WG Jr.. The effect of pressure and shear on autologous fat grafting. Plast Reconstr Surg. 2013;131:1125–1136.
Cheriyan T, Kao HK, Qiao X, Guo L. Low harvest pressure enhances autologous fat graft viability. Plast Reconstr Surg. 2014;133:1365–1368.
Del Vecchio DA, Del Vecchio SJ. The graft-to-capacity ratio: Volumetric planning in large-volume fat transplantation. Plast Reconstr Surg. 2014;133:561–569.
Boney CM, Moats-Staats BM, Stiles AD, D’Ercole AJ. Expression of insulin-like growth factor-I (IGF-I) and IGF-binding proteins during adipogenesis. Endocrinology 1994;135:1863–1868.
Cervelli V, Scioli MG, Gentile P, et al. Platelet-rich plasma greatly potentiates insulin-induced adipogenic differentiation of human adipose-derived stem cells through a serine/threonine kinase Akt-dependent mechanism and promotes clinical fat graft maintenance. Stem Cells Transl Med. 2012;1:206–220.
Gonzalez AM, Lobocki C, Kelly CP, Jackson IT. An alternative method for harvest and processing fat grafts: An in vitro study of cell viability and survival. Plast Reconstr Surg. 2007;120:285–294.
Kakudo N, Minakata T, Mitsui T, Kushida S, Notodihardjo FZ, Kusumoto K. Proliferation-promoting effect of platelet-rich plasma on human adipose-derived stem cells and human dermal fibroblasts. Plast Reconstr Surg. 2008;122:1352–1360.
Sommeling CE, Heyneman A, Hoeksema H, Verbelen J, Stillaert FB, Monstrey S. The use of platelet-rich plasma in plastic surgery: A systematic review. J Plast Reconstr Aesthet Surg. 2013;66:301–311.
Keerl S, Gehmert S, Gehmert S, Song YH, Alt E. PDGF and bFGF modulate tube formation in adipose tissue- derived stem cells. Ann Plast Surg. 2010;64:487–490.
Artemenko Y, Gagnon A, Aubin D, Sorisky A. Anti-adipogenic effect of PDGF is reversed by PKC inhibition. J Cell Physiol. 2005;204:646–653.
Kim YC, Mungunsukh O, McCart EA, Roehrich PJ, Yee DK, Day RM. Mechanism of erythropoietin regulation by angiotensin II. Mol Pharmacol. 2014;85:898–908.
Wu SK, Yang MT, Kang KH, et al. Targeted delivery of erythropoietin by transcranial focused ultrasound for neuroprotection against ischemia/reperfusion-induced neuronal injury: A long-term and short-term study. PLoS One 2014;9:e90107.
Hamed S, Bennett CL, Demiot C, Ullmann Y, Teot L, Desmoulière A. Erythropoietin, a novel repurposed drug: An innovative treatment for wound healing in patients with diabetes mellitus. Wound Repair Regen. 2014;22:23–33.
Hamed S, Egozi D, Kruchevsky D, Teot L, Gilhar A, Ullmann Y. Erythropoietin improves the survival of fat tissue after its transplantation in nude mice. PLoS One. 2010;5:e13986.
Jun-Jiang C, Huan-Jiu X. Vascular endothelial growth factor 165-transfected adipose-derived mesenchymal stem cells promote vascularization-assisted fat transplantation. Artif Cells Nanomed Biotechnol. 2016;44:1141–1149.
Ding SL, Zhang MY, Tang SJ, Yang H, Tan WQ. Effect of calcium alginate microsphere loaded with vascular endothelial growth factor on adipose tissue transplantation. Ann Plast Surg. 2015;75:644–651.
Li L, Pan S, Ni B, Lin Y. Improvement in autologous human fat transplant survival with SVF plus VEGF-PLA nano-sustained release microspheres. Cell Biol Int. 2014;38:962–970.
Zhang MY, Ding SL, Tang SJ, et al. Effect of chitosan nanospheres loaded with VEGF on adipose tissue transplantation: A preliminary report. Tissue Eng Part A 2014;20:2273–2282.
Tervala TV, Grönroos TJ, Hartiala P, et al. Analysis of fat graft metabolic adaptation and vascularization using positron emission tomography-computed tomographic imaging. Plast Reconstr Surg. 2014;133:291–299.
Chang L, Wang J, Zheng D, et al. Improvement of the survival of autologous free-fat transplants in rats using vascular endothelial growth factor 165-transfected bone mesenchymal stem cells. Ann Plast Surg. 2014;72:355–362.
Chung CW, Marra KG, Li H, et al. VEGF microsphere technology to enhance vascularization in fat grafting. Ann Plast Surg. 2012;69:213–219.
Topcu A, Aydin OE, Ünlü M, Barutcu A, Atabey A. Increasing the viability of fat grafts by vascular endothelial growth factor. Arch Facial Plast Surg. 2012;14:270–276.
Lu F, Li J, Gao J, et al. Improvement of the survival of human autologous fat transplantation by using VEGF-transfected adipose-derived stem cells. Plast Reconstr Surg. 2009;124:1437–1446.
Lei M, Liu SQ, Peng H, Liu YL. Effect of rhVEGF gene transfection on survival of grafts after autologous free granular fat transplantation in rats. Chin J Traumatol. 2008;11:49–53.
Yi CG, Xia W, Zhang LX, et al. VEGF gene therapy for the survival of transplanted fat tissue in nude mice. J Plast Reconstr Aesthet Surg. 2007;60:272–278.
Nishimura T, Hashimoto H, Nakanishi I, Furukawa M. Microvascular angiogenesis and apoptosis in the survival of free fat grafts. Laryngoscope 2000;110:1333–1338.
Jiang A, Li M, Duan W, Dong Y, Wang Y. Improvement of the survival of human autologous fat transplantation by adipose-derived stem-cells-assisted lipotransfer combined with bFGF. ScientificWorldJournal 2015;2015:968057.
Nakamura S, Ishihara M, Takikawa M, et al. Increased survival of free fat grafts and vascularization in rats with local delivery of fragmin/protamine microparticles containing FGF-2 (F/P MP-F). J Biomed Mater Res B Appl Biomater. 2011;96:234–241.
Hong SJ, Lee JH, Hong SM, Park CH. Enhancing the viability of fat grafts using new transfer medium containing insulin and beta-fibroblast growth factor in autologous fat transplantation. J Plast Reconstr Aesthet Surg. 2010;63:1202–1208.
Kuramochi D, Unoki H, Bujo H, et al. Matrix metalloproteinase 2 improves the transplanted adipocyte survival in mice. Eur J Clin Invest. 2008;38:752–759.
Marra KG, Defail AJ, Clavijo-Alvarez JA, et al. FGF-2 enhances vascularization for adipose tissue engineering. Plast Reconstr Surg. 2008;121:1153–1164.
Tamura E, Fukuda H, Tabata Y. Adipose tissue formation in response to basic fibroblast growth factor. Acta Otolaryngol. 2007;127:1327–1331.
Yazawa M, Mori T, Tuchiya K, Nakayama Y, Ogata H, Nakajima T. Influence of vascularized transplant bed on fat grafting. Wound Repair Regen. 2006;14:586–592.
Kimura Y, Ozeki M, Inamoto T, Tabata Y. Adipose tissue engineering based on human preadipocytes combined with gelatin microspheres containing basic fibroblast growth factor. Biomaterials 2003;24:2513–2521.
Eppley BL, Sidner RA, Platis JM, Sadove AM. Bioactivation of free-fat transfers: A potential new approach to improving graft survival. Plast Reconstr Surg. 1992;90:1022–1030.
Eppley BL, Snyders RV Jr, Winkelmann T, Delfino JJ. Autologous facial fat transplantation: Improved graft maintenance by microbead bioactivation. J Oral Maxillofac Surg. 1992;50:477–482.
Eppley BL, Sadove AM. A physicochemical approach to improving free fat graft survival: Preliminary observations. Aesthetic Plast Surg. 1991;15:215–218.
Yuksel E, Weinfeld AB, Cleek R, et al. Increased free fat-graft survival with the long-term, local delivery of insulin, insulin-like growth factor-I, and basic fibroblast growth factor by PLGA/PEG microspheres. Plast Reconstr Surg. 2000;105:1712–1720.
Park B, Kong JS, Kang S, Kim YW. The effect of epidermal growth factor on autogenous fat graft. Aesthetic Plast Surg. 2011;35:738–744.
Craft RO, Rophael J, Morrison WA, Vashi AV, Mitchell GM, Penington AJ. Effect of local, long-term delivery of platelet-derived growth factor (PDGF) on injected fat graft survival in severe combined immunodeficient (SCID) mice. J Plast Reconstr Aesthet Surg. 2009;62:235–243.
Fontdevila J, Guisantes E, Martínez E, Prades E, Berenguer J. Double-blind clinical trial to compare autologous fat grafts versus autologous fat grafts with PDGF: No effect of PDGF. Plast Reconstr Surg. 2014;134:219e–230e.
Sabbatini M, Bosetti M, Borrone A, et al. Erythropoietin stimulation of human adipose tissue for therapeutic refilling releases protective cytokines. J Tissue Eng. 2016;7:2041731416671278.
Sabbatini M, Moalem L, Bosetti M, et al. Effects of erythropoietin on adipose tissue: A possible strategy in refilling. Plast Reconstr Surg Glob Open 2015;3:e338.
Jin R, Zhang L, Zhang YG. Does platelet-rich plasma enhance the survival of grafted fat? An update review. Int J Clin Exp Med. 2013;6:252–258.
Liao HT, Marra KG, Rubin JP. Application of platelet-rich plasma and platelet-rich fibrin in fat grafting: Basic science and literature review. Tissue Eng Part B Rev. 2014;20:267–276.
Li J, Shi X, Chen W. [Influence of repeatedly injecting platelet-rich plasma on survival and quality of fat grafts in nude mice]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2013;27:454–459.
Sadati KS, Corrado AC, Alexander RW. Platelet-rich plasma (PRP) utilized to promote greater graft volume retention in autologous fat grafting. Am J Cosmetic Surg. 2006;4:627–631.
Gentile P, Di Pasquali C, Bocchini I, et al. Breast reconstruction with autologous fat graft mixed with platelet-rich plasma. Surg Innov. 2013;20:370–376.
Salgarello M, Visconti G, Rusciani A. Breast fat grafting with platelet-rich plasma: A comparative clinical study and current state of the art. Plast Reconstr Surg. 2011;127:2176–2185.
Li F, Guo W, Li K, et al. Improved fat graft survival by different volume fractions of platelet-rich plasma and adipose-derived stem cells. Aesthet Surg J. 2015;35:319–333.
Por YC, Yeow VK, Louri N, Lim TK, Kee I, Song IC. Platelet-rich plasma has no effect on increasing free fat graft survival in the nude mouse. J Plast Reconstr Aesthet Surg. 2009;62:1030–1034.
Nakamura S, Ishihara M, Takikawa M, et al. Platelet-rich plasma (PRP) promotes survival of fat-grafts in rats. Ann Plast Surg. 2010;65:101–106.
Liao HT, James IB, Marra KG, Rubin JP. The effects of platelet-rich plasma on cell proliferation and adipogenic potential of adipose-derived stem cells. Tissue Eng Part A 2015;21:2714–2722.
Sasaki GH. The safety and efficacy of cell-assisted fat grafting to traditional fat grafting in the anterior mid-face: An indirect assessment by 3D imaging. Aesthetic Plast Surg. 2015;39:833–846.
Tajima S, Tobita M, Orbay H, Hyakusoku H, Mizuno H. Direct and indirect effects of a combination of adipose-derived stem cells and platelet-rich plasma on bone regeneration. Tissue Eng Part A 2015;21:895–905.
Gentile P, Di Pasquali C, Bocchini I, et al. Breast reconstruction with autologous fat graft mixed with platelet-rich plasma. Surg Innov. 2013;20:370–376.
Keyhan SO, Hemmat S, Badri AA, Abdeshahzadeh A, Khiabani K. Use of platelet-rich fibrin and platelet-rich plasma in combination with fat graft: Which is more effective during facial lipostructure? J Oral Maxillofac Surg. 2013;71:610–621.
Niţă AC, Jianu DM, Florescu IP, et al. The synergy between lasers and adipose tissues surgery in cervicofacial rejuvenation: Histopathological aspects. Rom J Morphol Embryol. 2013;54:1039–1043.
Cervelli V, Nicoli F, Spallone D, et al. Treatment of traumatic scars using fat grafts mixed with platelet-rich plasma, and resurfacing of skin with the 1540 nm nonablative laser. Clin Exp Dermatol. 2012;37:55–61.
Gentile P, Orlandi A, Scioli MG, et al. A comparative translational study: The combined use of enhanced stromal vascular fraction and platelet-rich plasma improves fat grafting maintenance in breast reconstruction. Stem Cells Transl Med. 2012;1:341–351.
Salgarello M, Visconti G, Rusciani A. Breast fat grafting with platelet-rich plasma: A comparative clinical study and current state of the art. Plast Reconstr Surg. 2011;127:2176–2185.
Cervelli V, De Angelis B, Lucarini L, et al. Tissue regeneration in loss of substance on the lower limbs through use of platelet-rich plasma, stem cells from adipose tissue, and hyaluronic acid. Adv Skin Wound Care 2010;23:262–272.
Cervelli V, Gentile P, Grimaldi M. Regenerative surgery: Use of fat grafting combined with platelet-rich plasma for chronic lower-extremity ulcers. Aesthetic Plast Surg. 2009;33:340–345.
Pikuła M, Marek-Trzonkowska N, Wardowska A, Renkielska A, Trzonkowski P. Adipose tissue-derived stem cells in clinical applications. Expert Opin Biol Ther. 2013;13:1357–1370.
Kølle SF, Fischer-Nielsen A, Mathiasen AB, et al. Enrichment of autologous fat grafts with ex-vivo expanded adipose tissue-derived stem cells for graft survival: A randomised placebo-controlled trial. Lancet 2013;382:1113–1120.
Sterodimas A, de Faria J, Nicaretta B, Boriani F. Autologous fat transplantation versus adipose-derived stem cell-enriched lipografts: A study. Aesthet Surg J. 2011;31:682–693.
Tanikawa DY, Aguena M, Bueno DF, Passos-Bueno MR, Alonso N. Fat grafts supplemented with adipose-derived stromal cells in the rehabilitation of patients with craniofacial microsomia. Plast Reconstr Surg. 2013;132:141–152.
Begic A, Isfoss BL, Lønnerød LK, Vigen A, Moskaug JØ. Survival and inflammatory response in adipose-derived mesenchymal stem cell-enriched mouse fat grafts. Plast Reconstr Surg Glob Open 2016;4:e1110.
Luan A, Duscher D, Whittam AJ, et al. Cell-assisted lipotransfer improves volume retention in irradiated recipient sites and rescues radiation-induced skin changes. Stem Cells 2016;34:668–673.
Frazier TP, Bowles A, Lee S, et al. Serially transplanted nonpericytic CD146(-) adipose stromal/stem cells in silk bioscaffolds regenerate adipose tissue in vivo. Stem Cells 2016;34:1097–1111.
Ha KY, Park H, Park SH, et al. The relationship of a combination of human adipose tissue-derived stem cells and frozen fat with the survival rate of transplanted fat. Arch Plast Surg. 2015;42:677–685.
Jung DW, Kim YH, Kim TG, et al. Improvement of fat transplantation: Fat graft with adipose-derived stem cells and oxygen-generating microspheres. Ann Plast Surg. 2015;75:463–470.
Kashimura T, Soejima K, Asami T, Kazama T, Matsumoto T, Nakazawa H. The effect of mature adipocyte-derived dedifferentiated fat (DFAT) cells on a dorsal skin flap model. J Invest Surg. 2016;29:6–12.
Loder S, Peterson JR, Agarwal S, et al. Wound healing after thermal injury is improved by fat and adipose-derived stem cell isografts. J Burn Care Res. 2015;36:70–76.
Zhu M, Dong Z, Gao J, et al. Adipocyte regeneration after free fat transplantation: Promotion by stromal vascular fraction cells. Cell Transplant. 2015;24:49–62.
Derby BM, Dai H, Reichensperger J, et al. Adipose-derived stem cell to epithelial stem cell transdifferentiation: A mechanism to potentially improve understanding of fat grafting’s impact on skin rejuvenation. Aesthet Surg J. 2014;34:142–153.
Koellensperger E, Lampe K, Beierfuss A, Gramley F, Germann G, Leimer U. Intracutaneously injected human adipose tissue-derived stem cells in a mouse model stay at the site of injection. J Plast Reconstr Aesthet Surg. 2014;67:844–850.
Kono S, Kazama T, Kano K, Harada K, Uechi M, Matsumoto T. Phenotypic and functional properties of feline dedifferentiated fat cells and adipose-derived stem cells. Vet J. 2014;199:88–96.
Tian T, Jia C, Liu Y, et al. [Effects of rat allogeneic adipose-derived stem cells on the early neovascularization of autologous fat transplantation]. Zhonghua Shao Shang Za Zhi 2014;30:512–517.
Trivisonno A, Di Rocco G, Cannistra C, et al. Harvest of superficial layers of fat with a microcannula and isolation of adipose tissue-derived stromal and vascular cells. Aesthet Surg J. 2014;34:601–613.
Dong Z, Peng Z, Chang Q, Lu F. The survival condition and immunoregulatory function of adipose stromal vascular fraction (SVF) in the early stage of nonvascularized adipose transplantation. PLoS One 2013;8:e80364.
He X, Zhong X, Ni Y, Liu M, Liu S, Lan X. Effect of ASCs on the graft survival rates of fat particles in rabbits. J Plast Surg Hand Surg. 2013;47:3–7.
Lee JY, Lee KH, Shin HM, Chung KH, Kim GI, Lew H. Orbital volume augmentation after injection of human orbital adipose-derived stem cells in rabbits. Invest Ophthalmol Vis Sci. 2013;54:2410–2416.
Liu B, Tan XY, Liu YP, et al. The adjuvant use of stromal vascular fraction and platelet-rich fibrin for autologous adipose tissue transplantation. Tissue Eng Part C Methods 2013;19:1–14.
Philips BJ, Grahovac TL, Valentin JE, et al. Prevalence of endogenous CD34+ adipose stem cells predicts human fat graft retention in a xenograft model. Plast Reconstr Surg. 2013;132:845–858.
Wang L, Johnson JA, Zhang Q, Beahm EK. Combining decellularized human adipose tissue extracellular matrix and adipose-derived stem cells for adipose tissue engineering. Acta Biomater. 2013;9:8921–8931.
Zamperone A, Pietronave S, Merlin S, et al. Isolation and characterization of a spontaneously immortalized multipotent mesenchymal cell line derived from mouse subcutaneous adipose tissue. Stem Cells Dev. 2013;22:2873–2884.
Zhu W, Shi XL, Xiao JQ, Gu GX, Ding YT, Ma ZL. Effects of xenogeneic adipose-derived stem cell transplantation on acute-on-chronic liver failure. Hepatobiliary Pancreat Dis Int. 2013;12:60–67.
Zografou A, Papadopoulos O, Tsigris C, et al. Autologous transplantation of adipose-derived stem cells enhances skin graft survival and wound healing in diabetic rats. Ann Plast Surg. 2013;71:225–232.
Butler MJ, Sefton MV. Cotransplantation of adipose-derived mesenchymal stromal cells and endothelial cells in a modular construct drives vascularization in SCID/bg mice. Tissue Eng Part A 2012;18:1628–1641.
Venugopal B, Fernandez FB, Babu SS, Harikrishnan VS, Varma H, John A. Adipogenesis on biphasic calcium phosphate using rat adipose-derived mesenchymal stem cells: In vitro and in vivo. J Biomed Mater Res A 2012;100:1427–1437.
Li GZ, Sun QZ, Xiong ZY, Huang H, Xu J. [The effect of adipose-derived stem cells on viability of random pattern skin flap in rabbits]. Zhonghua Zheng Xing Wai Ke Za Zhi 2011;27:119–123.
Ko MS, Jung JY, Shin IS, et al. Effects of expanded human adipose tissue-derived mesenchymal stem cells on the viability of cryopreserved fat grafts in the nude mouse. Int J Med Sci. 2011;8:231–238.
Fu BC, Gao JH, Lu F, Li J. [Experimental study of the effect of adipose stromal vascular fraction cells on the survival rate of fat transplantation]. Zhonghua Zheng Xing Wai Ke Za Zhi 2010;26:289–294.
Moyer HR, Kinney RC, Singh KA, Williams JK, Schwartz Z, Boyan BD. Alginate microencapsulation technology for the percutaneous delivery of adipose-derived stem cells. Ann Plast Surg. 2010;65:497–503.
Jiang SJ, He XS, Ni YD, Liu ML, Liu SH, Zhong XC. [Proliferation of the mesenchymal stem cells in a delayed fat flap: an experimental study in rabbits]. Zhonghua Zheng Xing Wai Ke Za Zhi 2009;25:287–289.
Li J, Gao JH, Lu F, Li HM, Fu BC. [Experimental study of the effect of adipose tissue derived stem cells on the survival rate of free fat transplantation]. Zhonghua Zheng Xing Wai Ke Za Zhi 2009;25:129–133.
Li H, Gao J, Lu F, Li H, Chen X, Fu B. [Comparison between kinds of myofascial flap encapsulating adipose-derived stromal cells carrier complex in terms of adipogenic efficacy in vivo]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2009;23:161–165.
Lu F, Li J, Gao J, et al. Improvement of the survival of human autologous fat transplantation by using VEGF-transfected adipose-derived stem cells. Plast Reconstr Surg. 2009;124:1437–1446.
Yoo G, Lim JS. Tissue engineering of injectable soft tissue filler: Using adipose stem cells and micronized acellular dermal matrix. J Korean Med Sci. 2009;24:104–109.
Lu F, Mizuno H, Uysal CA, Cai X, Ogawa R, Hyakusoku H. Improved viability of random pattern skin flaps through the use of adipose-derived stem cells. Plast Reconstr Surg. 2008;121:50–58.
Mizuno H, Itoi Y, Kawahara S, Ogawa R, Akaishi S, Hyakusoku H. In vivo adipose tissue regeneration by adipose-derived stromal cells isolated from GFP transgenic mice. Cells Tissues Organs. 2008;187:177–185.
Matsumoto D, Sato K, Gonda K, et al. Cell-assisted lipotransfer: Supportive use of human adipose-derived cells for soft tissue augmentation with lipoinjection. Tissue Eng. 2006;12:3375–3382.
Clavijo-Alvarez JA, Rubin JP, Bennett J, et al. A novel perfluoroelastomer seeded with adipose-derived stem cells for soft-tissue repair. Plast Reconstr Surg. 2006;118:1132–1142; discussion 1143.
Hong L, Peptan IA, Colpan A, Daw JL. Adipose tissue engineering by human adipose-derived stromal cells. Cells Tissues Organs 2006;183:133–140.
Dos Anjos S, Matas-Palau A, Mercader J, Katz AJ, Llull R. Reproducible volume restoration and efficient long-term volume retention after point-of-care standardized cell-enhanced fat grafting in breast surgery. Plast Reconstr Surg Glob Open 2015;3:e547.
Charles-de-Sa L, Gontijo-de-Amorim NF, Maeda Takiya C, et al. Antiaging treatment of the facial skin by fat graft and adipose-derived stem cells. Plast Reconstr Surg. 2015;135:999–1009.
Hanson SE, Kim J, Hematti P. Comparative analysis of adipose-derived mesenchymal stem cells isolated from abdominal and breast tissue. Aesthet Surg J. 2013;33:888–898.
Marino G, Moraci M, Armenia E, et al. Therapy with autologous adipose-derived regenerative cells for the care of chronic ulcer of lower limbs in patients with peripheral arterial disease. J Surg Res. 2013;185:36–44.
Tanikawa DY, Aguena M, Bueno DF, Passos-Bueno MR, Alonso N. Fat grafts supplemented with adipose-derived stromal cells in the rehabilitation of patients with craniofacial microsomia. Plast Reconstr Surg. 2013;132:141–152.
Doornaert MA, Declercq H, Stillaert F, et al. Intrinsic dynamics of the fat graft: In vitro interactions between the main cell actors. Plast Reconstr Surg. 2012;130:1001–1009.
Koh KS, Oh TS, Kim H, et al. Clinical application of human adipose tissue-derived mesenchymal stem cells in progressive hemifacial atrophy (Parry-Romberg disease) with microfat grafting techniques using 3-dimensional computed tomography and 3-dimensional camera. Ann Plast Surg. 2012;69:331–337.
Sterodimas A, de Faria J, Nicaretta B, Boriani F. Autologous fat transplantation versus adipose-derived stem cell-enriched lipografts: A study. Aesthet Surg J. 2011;31:682–693.
Tiryaki T, Findikli N, Tiryaki D. Staged stem cell-enriched tissue (SET) injections for soft tissue augmentation in hostile recipient areas: A preliminary report. Aesthetic Plast Surg. 2011;35:965–971.
Yoshimura K, Sato K, Aoi N, Kurita M, Hirohi T, Harii K. Cell-assisted lipotransfer for cosmetic breast augmentation: Supportive use of adipose-derived stem/stromal cells. Aesthetic Plast Surg. 2008;32:48–55; discussion 56.
Yi CG, Xia W, Zhang LX, et al. VEGF gene therapy for the survival of transplanted fat tissue in nude mice. J Plast Reconstr Aesthet Surg. 2007;60:272–278.
Jia F, Wilson KD, Sun N, et al. A nonviral minicircle vector for deriving human iPS cells. Nat Methods 2010;7:197–199.
Hyun J, Grova M, Nejadnik H, et al. Enhancing in vivo survival of adipose-derived stromal cells through Bcl-2 overexpression using a minicircle vector. Stem Cells Transl Med. 2013;2:690–702.
Keeney M, Chung MT, Zielins ER, et al. Scaffold-mediated BMP-2 minicircle DNA delivery accelerated bone repair in a mouse critical-size calvarial defect model. J Biomed Mater Res A 2016;104:2099–2107.
Huang M, Nguyen P, Jia F, et al. Double knockdown of prolyl hydroxylase and factor-inhibiting hypoxia-inducible factor with nonviral minicircle gene therapy enhances stem cell mobilization and angiogenesis after myocardial infarction. Circulation 2011;124(11 Suppl):S46–S54.
Baldari S, Di Rocco G, Trivisonno A, Samengo D, Pani G, Toietta G. Promotion of survival and engraftment of transplanted adipose tissue-derived stromal and vascular cells by overexpression of manganese superoxide dismutase. Int J Mol Sci. 2016;17(7).
Lequeux C, Oni G, Wong C, et al. Subcutaneous fat tissue engineering using autologous adipose-derived stem cells seeded onto a collagen scaffold. Plast Reconstr Surg. 2012;130:1208–1217.
Morselli PG, Giorgini FA, Pazzini C, Muscari C. Lull pgm system: A suitable technique to improve the regenerative potential of autologous fat grafting. Wound Repair Regen. 2017;25:722–729.
Sarfati I, van la Parra RFD, Terem-Rapoport CA, Benyahi D, Nos C, Clough KB. A prospective randomized study comparing centrifugation and sedimentation for fat grafting in breast reconstruction. J Plast Reconstr Aesthet Surg. 2017;70:1218–1228.
Gassman AA, Lewis MS, Lee JC. Remote ischemic preconditioning recipient tissues improves the viability of murine fat transfer. Plast Reconstr Surg. 2016;138:55e–63e.
Qiu L, Su Y, Zhang D, et al. Identification of the centrifuged lipoaspirate fractions suitable for postgrafting survival. Plast Reconstr Surg. 2016;137:67e–76e.
Cucchiani R, Corrales L. The effects of fat harvesting and preparation, air exposure, obesity, and stem cell enrichment on adipocyte viability prior to graft transplantation. Aesthet Surg J. 2016;36:1164–1173.
Lui YF, Ip WY. Application of hydrogel in reconstruction surgery: hydrogel/fat graft complex filler for volume reconstruction in critical sized muscle defects. Biomed Res Int. 2016;2016:3459431.
Reddy R, Iyer S, Sharma M, et al. Effect of external volume expansion on the survival of fat grafts. Indian J Plast Surg. 2016;49:151–158.
Yuan Y, Zhang S, Gao J, Lu F. Spatial structural integrity is important for adipose regeneration after transplantation. Arch Dermatol Res. 2015;307:693–704.
Bae YC, Song JS, Bae SH, Kim JH. Effects of human adipose-derived stem cells and stromal vascular fraction on cryopreserved fat transfer. Dermatol Surg. 2015;41:605–614.
Garza RM, Rennert RC, Paik KJ, et al. Studies in fat grafting: Part IV. Adipose-derived stromal cell gene expression in cell-assisted lipotransfer. Plast Reconstr Surg. 2015;135:1045–1055.
Gillis J, Gebremeskel S, Phipps KD, et al. Effect of N-acetylcysteine on adipose-derived stem cell and autologous fat graft survival in a mouse model. Plast Reconstr Surg. 2015;136:179e–188e.
Li SL, Liu Y, Hui L. Construction of engineering adipose-like tissue in vivo utilizing human insulin gene-modified umbilical cord mesenchymal stromal cells with silk fibroin 3D scaffolds. J Tissue Eng Regen Med. 2015;9:E267–E275.
Luo X, Cao W, Xu H, et al. Coimplanted endothelial cells improve adipose tissue grafts’ survival by increasing vascularization. J Craniofac Surg. 2015;26:358–364.
Osinga R, Menzi NR, Tchang LA, et al. Effects of intersyringe processing on adipose tissue and its cellular components: Implications in autologous fat grafting. Plast Reconstr Surg. 2015;135:1618–1628.
Phipps KD, Gebremeskel S, Gillis J, Hong P, Johnston B, Bezuhly M. Alternatively activated M2 macrophages improve autologous fat graft survival in a mouse model through induction of angiogenesis. Plast Reconstr Surg. 2015;135:140–149.
Soares MA, Ezeamuzie OC, Ham MJ, et al. Targeted protection of donor graft vasculature using a phosphodiesterase inhibitor increases survival and predictability of autologous fat grafts. Plast Reconstr Surg. 2015;135:488–499.
Yu L, Zhang R, Li P, et al. Traditional Chinese medicine: Salvia miltiorrhiza enhances survival rate of autologous adipose tissue transplantation in rabbit model. Aesthetic Plast Surg. 2015;39:985–991.
Beitzel K, McCarthy MB, Cote MP, et al. Properties of biologic scaffolds and their response to mesenchymal stem cells. Arthroscopy 2014;30:289–298.
Chung MT, Paik KJ, Atashroo DA, et al. Studies in fat grafting: Part I. Effects of injection technique on in vitro fat viability and in vivo volume retention. Plast Reconstr Surg. 2014;134:29–38.
Saliba I, Alzahrani M, Zhu T, Chemtob S. Growth factors expression in hyaluronic acid fat graft myringoplasty. Laryngoscope 2014;124:E224–E230.
Sezgin B, Ozmen S, Bulam H, et al. Improving fat graft survival through preconditioning of the recipient site with microneedling. J Plast Reconstr Aesthet Surg. 2014;67:712–720.
Willemsen JC, van der Lei B, Vermeulen KM, Stevens HP. The effects of platelet-rich plasma on recovery time and aesthetic outcome in facial rejuvenation: Preliminary retrospective observations. Aesthetic Plast Surg. 2014;38:1057–1063.
Xu FT, Li HM, Yin QS, et al. Human breast adipose-derived stem cells transfected with the stromal cell-derived factor-1 receptor CXCR4 exhibit enhanced viability in human autologous free fat grafts. Cell Physiol Biochem. 2014;34:2091–2104.
Zhou SB, Chiang CA, Xie Y, et al. In vivo bioimaging analysis of stromal vascular fraction-assisted fat grafting: The interaction and mutualism of cells and grafted fat. Transplantation 2014;98:1048–1055.
Aronowitz JA, Ellenhorn JD. Adipose stromal vascular fraction isolation: A head-to-head comparison of four commercial cell separation systems. Plast Reconstr Surg. 2013;132:932e–939e.
Bulgin D, Vrabic E, Hodzic E. Autologous bone-marrow-derived-mononuclear-cells-enriched fat transplantation in breast augmentation: Evaluation of clinical outcomes and aesthetic results in a 30-year-old female. Case Rep Surg. 2013;2013:782069.
Yanaga H, Imai K, Tanaka Y, Yanaga K. Two-stage transplantation of cell-engineered autologous auricular chondrocytes to regenerate chondrofat composite tissue: Clinical application in regenerative surgery. Plast Reconstr Surg. 2013;132:1467–1477.
Zhao J, Yi C, Zheng Y, et al. Enhancement of fat graft survival by bone marrow-derived mesenchymal stem cell therapy. Plast Reconstr Surg. 2013;132:1149–1157.
Alghoul M, Mendiola A, Seth R, et al. The effect of hyaluronan hydrogel on fat graft survival. Aesthet Surg J. 2012;32:622–633.
Butala P, Hazen A, Szpalski C, Sultan SM, Coleman SR, Warren SM. Endogenous stem cell therapy enhances fat graft survival. Plast Reconstr Surg. 2012;130:293–306.
Frerich B, Winter K, Scheller K, Braumann UD. Comparison of different fabrication techniques for human adipose tissue engineering in severe combined immunodeficient mice. Artif Organs 2012;36:227–237.
Lee SK, Kim DW, Dhong ES, Park SH, Yoon ES. Facial soft tissue augmentation using autologous fat mixed with stromal vascular fraction. Arch Plast Surg. 2012;39:534–539.
Ma Z, Han D, Zhang P, et al. Utilizing muscle-derived stem cells to enhance long-term retention and aesthetic outcome of autologous fat grafting: Pilot study in mice. Aesthetic Plast Surg. 2012;36:186–192.
Sarkanen JR, Ruusuvuori P, Kuokkanen H, Paavonen T, Ylikomi T. Bioactive acellular implant induces angiogenesis and adipogenesis and sustained soft tissue restoration in vivo. Tissue Eng Part A 2012;18:2568–2580.
Yang M, Zhang F, Sailes FC, Zhang EW, Lin S, Das SK. Role of anti-TNF-α therapy in fat graft preservation. Ann Plast Surg. 2012;68:531–535.
Keck M, Zeyda M, Burjak S, et al. Coenzyme Q10 does not enhance preadipocyte viability in an in vitro lipotransfer model. Aesthetic Plast Surg. 2012;36:453–457.
Koh YJ, Koh BI, Kim H, et al. Stromal vascular fraction from adipose tissue forms profound vascular network through the dynamic reassembly of blood endothelial cells. Arterioscler Thromb Vasc Biol. 2011;31:1141–1150.
Mojallal A, Lequeux C, Shipkov C, et al. Stem cells, mature adipocytes, and extracellular scaffold: What does each contribute to fat graft survival? Aesthetic Plast Surg. 2011;35:1061–1072.
Kamakura T, Ito K. Autologous cell-enriched fat grafting for breast augmentation. Aesthetic Plast Surg. 2011;35:1022–1030.
Conde-Green A, Baptista LS, de Amorin NF, et al. Effects of centrifugation on cell composition and viability of aspirated adipose tissue processed for transplantation. Aesthet Surg J. 2010;30:249–55.
Keck M, Zeyda M, Gollinger K, et al. Local anesthetics have a major impact on viability of preadipocytes and their differentiation into adipocytes. Plast Reconstr Surg. 2010;126:1500–1505.
Zhu M, Zhou Z, Chen Y, et al. Supplementation of fat grafts with adipose-derived regenerative cells improves long-term graft retention. Ann Plast Surg. 2010;64:222–228.
Umeno H, Chitose S, Murofushi Y, et al. Efficacy of autologous fat injection laryngoplasty with an adenoviral vector expressing hepatocyte growth factor in a canine model. J Laryngol Otol Suppl. 2009;31:24–29.
Zhong X, Yan W, He X, Ni Y. Improved fat graft viability by delayed fat flap with ischaemic pretreatment. J Plast Reconstr Aesthet Surg. 2009;62:526–531.
Mojallal A, Lequeux C, Auxenfans C, Braye F, Damour O. Does adipose tissue cultured with collagen matrix and preadipocytes give comparable results to the standard technique in plastic surgery? Biomed Mater Eng. 2008;18:187–192.
Piasecki JH, Gutowski KA, Moreno KM, Lahvis GL. Purified viable fat suspended in matrigel improves volume longevity. Aesthet Surg J. 2008;28:24–32.
Piasecki JH, Moreno K, Gutowski KA. Beyond the cells: Scaffold matrix character affects the in vivo performance of purified adipocyte fat grafts. Aesthet Surg J. 2008;28:306–312.
Torio-Padron N, Baerlecken N, Momeni A, Stark GB, Borges J. Engineering of adipose tissue by injection of human preadipocytes in fibrin. Aesthetic Plast Surg. 2007;31:285–293.
Shoshani O, Livne E, Armoni M, et al. The effect of interleukin-8 on the viability of injected adipose tissue in nude mice. Plast Reconstr Surg. 2005;115:853–859.
Yamaguchi M, Matsumoto F, Bujo H, et al. Revascularization determines volume retention and gene expression by fat grafts in mice. Exp Biol Med (Maywood) 2005;230:742–748.
Moore JH Jr, Kolaczynski JW, Morales LM, et al. Viability of fat obtained by syringe suction lipectomy: Effects of local anesthesia with lidocaine. Aesthetic Plast Surg. 1995;19:335–339.
Sabbatini M, Moalem L, Bosetti M, et al. Effects of erythropoietin on adipose tissue: A possible strategy in refilling. Plast Reconstr Surg Glob Open 2015;3:e338.
Sasaki GH. The safety and efficacy of cell-assisted fat grafting to traditional fat grafting in the anterior mid-face: An indirect assessment by 3D imaging. Aesthetic Plast Surg. 2015;39:833–846.