Characterising viable virus from air exhaled by H1N1 influenza-infected ferrets reveals the importance of haemagglutinin stability for airborne infectivity.
Air
/ analysis
Air Microbiology
Animals
Cell Line
Disease Transmission, Infectious
Ferrets
/ virology
Hemagglutinin Glycoproteins, Influenza Virus
/ genetics
Hemagglutinins
/ immunology
Humans
Influenza A Virus, H1N1 Subtype
/ metabolism
Influenza, Human
/ virology
Microbial Viability
/ immunology
Orthomyxoviridae Infections
/ virology
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
01
07
2019
accepted:
28
01
2020
revised:
06
03
2020
pubmed:
26
2
2020
medline:
6
6
2020
entrez:
26
2
2020
Statut:
epublish
Résumé
The transmissibility and pandemic potential of influenza viruses depends on their ability to efficiently replicate and be released from an infected host, retain viability as they pass through the environment, and then initiate infection in the next host. There is a significant gap in knowledge about viral properties that enable survival of influenza viruses between hosts, due to a lack of experimental methods to reliably isolate viable virus from the air. Using a novel technique, we isolate and characterise infectious virus from droplets emitted by 2009 pandemic H1N1-infected ferrets. We demonstrate that infectious virus is predominantly released early after infection. A virus containing a mutation destabilising the haemagglutinin (HA) surface protein displayed reduced survival in air. Infectious virus recovered from droplets exhaled by ferrets inoculated with this virus contained mutations that conferred restabilisation of HA, indicating the importance of influenza HA stability for between-host survival. Using this unique approach can improve knowledge about the determinants and mechanisms of influenza transmissibility and ultimately could be applied to studies of airborne virus exhaled from infected people.
Identifiants
pubmed: 32097448
doi: 10.1371/journal.ppat.1008362
pii: PPATHOGENS-D-19-01205
pmc: PMC7059951
doi:
Substances chimiques
H1N1 virus hemagglutinin
0
Hemagglutinin Glycoproteins, Influenza Virus
0
Hemagglutinins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1008362Subventions
Organisme : National Centre for the Replacement, Refinement and Reduction of Animals in Research
ID : G1000033/1
Pays : United Kingdom
Organisme : National Centre for the Replacement, Refinement and Reduction of Animals in Research
ID : NC/K00042X/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 200187/Z/15/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : WT105736MA
Pays : United Kingdom
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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