Improving the etiological diagnosis of osteoarticular infections with the commercial multiplex real-time polymerase chain reaction SeptiFast®.
Anti-Bacterial Agents
/ therapeutic use
Arthritis, Infectious
/ diagnosis
Bacteria
/ drug effects
Bacterial Infections
/ diagnosis
Biopsy
Humans
Multiplex Polymerase Chain Reaction
Predictive Value of Tests
Prostheses and Implants
/ microbiology
Reagent Kits, Diagnostic
Sensitivity and Specificity
Spine
/ pathology
Synovial Fluid
/ microbiology
Osteoarticular infections
Real-time PCR
SeptiFast
Synovial fluid
Journal
Diagnostic microbiology and infectious disease
ISSN: 1879-0070
Titre abrégé: Diagn Microbiol Infect Dis
Pays: United States
ID NLM: 8305899
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
25
09
2019
revised:
20
12
2019
accepted:
26
01
2020
pubmed:
27
2
2020
medline:
11
11
2020
entrez:
27
2
2020
Statut:
ppublish
Résumé
Detection of etiological agents is pivotal for adequate therapy of osteoarticular bacterial infections. Culture often lacks sensitivity, especially in patients under antibiotic therapy. The present study investigates the potential clinical utility of the commercial multiplex real-time polymerase chain reaction SeptiFast® (SF) in the etiological diagnosis of osteoarticular infections. Results obtained from conventional culture and SF were compared in 86 osteoarticular specimens collected from patients with suspected infection. The number of specimens positive by SF (38/86, 44.18%) was significantly greater (P = 0.001) than that of specimens positive by culture (20/86, 23.25%). The sensitivity of SF was 48.71%, significantly higher than culture sensitivity (25.64%). Specificity was 100% for both tests. The overall diagnostic accuracy for SF was 53.48%, and that of culture was 32.55%. Even with the limitation of the low number of specimens, this study supports the usefulness of SF in the diagnosis of osteoarticular infections.
Identifiants
pubmed: 32098689
pii: S0732-8893(19)30992-7
doi: 10.1016/j.diagmicrobio.2020.115002
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Reagent Kits, Diagnostic
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
115002Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.