Serine-Arginine Protein Kinase 1 Regulates Ebola Virus Transcription.
Africa, Western
Animals
Cell Line
Cell Survival
Chlorocebus aethiops
Ebolavirus
/ genetics
HEK293 Cells
Hemorrhagic Fever, Ebola
/ virology
Humans
Inclusion Bodies, Viral
/ metabolism
Nucleoproteins
Phosphorylation
Protein Serine-Threonine Kinases
/ metabolism
RNA, Viral
/ genetics
Transcription Factors
/ metabolism
Vero Cells
Viral Proteins
/ metabolism
Virus Replication
/ genetics
Ebola virus
VP30
kinase
protein phosphorylation
transcription
Journal
mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231
Informations de publication
Date de publication:
25 02 2020
25 02 2020
Historique:
entrez:
27
2
2020
pubmed:
27
2
2020
medline:
25
8
2020
Statut:
epublish
Résumé
Ebola virus (EBOV) causes a severe and often fatal disease for which no approved vaccines or antivirals are currently available. EBOV VP30 has been described as a viral phosphoprotein, and nonphosphorylated VP30 is essential and sufficient to support secondary transcription in an EBOV-specific minigenome system; however, phosphorylatable serine residues near the N terminus of VP30 are required to support primary viral transcription as well as the reinitiation of VP30-mediated transcription at internal EBOV genes. While the dephosphorylation of VP30 by the cellular phosphatase PP2A was found to be mediated by nucleoprotein, the VP30-specific kinases and the role of phosphorylation remain unknown. Here, we report that serine-arginine protein kinase 1 (SRPK1) and SRPK2 phosphorylate serine 29 of VP30, which is located in an N-terminal R
Identifiants
pubmed: 32098814
pii: mBio.02565-19
doi: 10.1128/mBio.02565-19
pmc: PMC7042693
pii:
doi:
Substances chimiques
Nucleoproteins
0
RNA, Viral
0
Transcription Factors
0
Viral Proteins
0
SRPK1 protein, human
EC 2.7.1.-
Protein Serine-Threonine Kinases
EC 2.7.11.1
SRPK2 protein, human
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2020 Takamatsu et al.
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