Anti-Leishmanial and Immunomodulatory Effects of Epigallocatechin 3-O-Gallate on
Apoptosis
Epigallocatechin 3-O-gallate
Gene expression
Immunomodulatory role
Leishmania tropica
Journal
Iranian journal of parasitology
ISSN: 1735-7020
Titre abrégé: Iran J Parasitol
Pays: Iran
ID NLM: 101464309
Informations de publication
Date de publication:
Historique:
entrez:
27
2
2020
pubmed:
27
2
2020
medline:
27
2
2020
Statut:
ppublish
Résumé
Pentavalent antimonials such as meglumine antimoniate (MA, Glucantime), are the first-line treatment against leishmaniasis, but at present, they have basically lost their efficacy. This study was aimed to explore epigallocatechin 3-O-gallate (EGCG), alone or in combination with MA against All experiments were carried out in triplicate using colorimetric assay, macrophage model, flow cytometry and quantitative real-time PCR. This experimental study was carried out in 2017 in Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran. Promastigotes and amastigotes were more susceptible to EGCG than MA alone, but the effect was more profound when used in combination. EGCG exhibited high antioxidant level with a remarkable potential to induce apoptosis. Furthermore, the results showed that the level of gene expression pertaining to Th-1 was significantly up-regulated ( EGCG demonstrated a potent anti-leishmanial effect alone and more enhanced lethal activity in combination. The principal mode of action entails the stimulation of a synergistic response and up-regulation of the immunomodulatory role towards Th-1 response against
Sections du résumé
BACKGROUND
BACKGROUND
Pentavalent antimonials such as meglumine antimoniate (MA, Glucantime), are the first-line treatment against leishmaniasis, but at present, they have basically lost their efficacy. This study was aimed to explore epigallocatechin 3-O-gallate (EGCG), alone or in combination with MA against
METHODS
METHODS
All experiments were carried out in triplicate using colorimetric assay, macrophage model, flow cytometry and quantitative real-time PCR. This experimental study was carried out in 2017 in Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.
RESULTS
RESULTS
Promastigotes and amastigotes were more susceptible to EGCG than MA alone, but the effect was more profound when used in combination. EGCG exhibited high antioxidant level with a remarkable potential to induce apoptosis. Furthermore, the results showed that the level of gene expression pertaining to Th-1 was significantly up-regulated (
CONCLUSION
CONCLUSIONS
EGCG demonstrated a potent anti-leishmanial effect alone and more enhanced lethal activity in combination. The principal mode of action entails the stimulation of a synergistic response and up-regulation of the immunomodulatory role towards Th-1 response against
Types de publication
Journal Article
Langues
eng
Pagination
521-533Informations de copyright
Copyright© Iranian Society of Parasitology & Tehran University of Medical Sciences.
Déclaration de conflit d'intérêts
Conflict of interests The authors declare that there is no conflict of interests.
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