Mouse Heterochromatin Adopts Digital Compaction States without Showing Hallmarks of HP1-Driven Liquid-Liquid Phase Separation.
Heterochromatin protein 1
chromatin accessibility
chromatin compartmentalization
epigenetic editing
intracellular viscosity
liquid- liquid phase separation
nuclear organization
optodroplets
polarization-dependent fluorescence correlation spectroscopy
polymer collapse
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
16 04 2020
16 04 2020
Historique:
received:
07
08
2019
revised:
20
12
2019
accepted:
04
02
2020
pubmed:
27
2
2020
medline:
14
8
2020
entrez:
27
2
2020
Statut:
ppublish
Résumé
The formation of silenced and condensed heterochromatin foci involves enrichment of heterochromatin protein 1 (HP1). HP1 can bridge chromatin segments and form liquid droplets, but the biophysical principles underlying heterochromatin compartmentalization in the cell nucleus are elusive. Here, we assess mechanistically relevant features of pericentric heterochromatin compaction in mouse fibroblasts. We find that (1) HP1 has only a weak capacity to form liquid droplets in living cells; (2) the size, global accessibility, and compaction of heterochromatin foci are independent of HP1; (3) heterochromatin foci lack a separated liquid HP1 pool; and (4) heterochromatin compaction can toggle between two "digital" states depending on the presence of a strong transcriptional activator. These findings indicate that heterochromatin foci resemble collapsed polymer globules that are percolated with the same nucleoplasmic liquid as the surrounding euchromatin, which has implications for our understanding of chromatin compartmentalization and its functional consequences.
Identifiants
pubmed: 32101700
pii: S1097-2765(20)30075-7
doi: 10.1016/j.molcel.2020.02.005
pmc: PMC7163299
pii:
doi:
Substances chimiques
Chromatin
0
Chromosomal Proteins, Non-Histone
0
Euchromatin
0
Heterochromatin
0
Chromobox Protein Homolog 5
107283-02-3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
236-249.e7Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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