Glycosaminoglycan Domain Mapping of Cellular Chondroitin/Dermatan Sulfates.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
26 02 2020
26 02 2020
Historique:
received:
11
10
2019
accepted:
12
02
2020
entrez:
28
2
2020
pubmed:
28
2
2020
medline:
25
11
2020
Statut:
epublish
Résumé
Glycosaminoglycans (GAGs) are polysaccharides produced by most mammalian cells and involved in a variety of biological processes. However, due to the size and complexity of GAGs, detailed knowledge about the structure and expression of GAGs by cells, the glycosaminoglycome, is lacking. Here we report a straightforward and versatile approach for structural domain mapping of complex mixtures of GAGs, GAGDoMa. The approach is based on orthogonal enzymatic depolymerization of the GAGs to generate internal, terminating, and initiating domains, and nanoflow reversed-phase ion-pairing chromatography with negative mode higher-energy collision dissociation (HCD) tandem mass spectrometry (MS/MS) for structural characterization of the individual domains. GAGDoMa provides a detailed structural insight into the glycosaminoglycome, and offers an important tool for deciphering the complexity of GAGs in cellular physiology and pathology.
Identifiants
pubmed: 32103093
doi: 10.1038/s41598-020-60526-0
pii: 10.1038/s41598-020-60526-0
pmc: PMC7044218
doi:
Substances chimiques
Glycosaminoglycans
0
Dermatan Sulfate
24967-94-0
Chondroitin Sulfates
9007-28-7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3506Références
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