Comparison of microbiota, antimicrobial resistance genes and mobile genetic elements in flies and the feces of sympatric animals.
antimicrobial resistance genes
feces
flies
microbiota
mobile genetic elements
surveillance
Journal
FEMS microbiology ecology
ISSN: 1574-6941
Titre abrégé: FEMS Microbiol Ecol
Pays: England
ID NLM: 8901229
Informations de publication
Date de publication:
01 04 2020
01 04 2020
Historique:
received:
26
09
2019
accepted:
25
02
2020
pubmed:
28
2
2020
medline:
21
11
2020
entrez:
28
2
2020
Statut:
ppublish
Résumé
Flies are well-known vectors of bacterial pathogens, but there are little data on their role in spreading microbial community and antimicrobial resistance. In this study, we compared the bacterial community, antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs) in flies with those in the feces of sympatric animals. A 16S rRNA-based microbial analysis identified 23 bacterial phyla in fecal samples and 25 phyla in flies; all the phyla identified in the fecal samples were also found in the flies. Bray-Curtis dissimilarity analysis showed that the microbiota of the flies were more similar to the microbiota of the feces of their sympatric animals than those of the feces from the three other animal species studied. The qPCR array amplified 276 ARGs/MGEs in fecal samples, and 216 ARGs/MGEs in the flies, while 198 of these genes were identified in both flies and feces. Long-term studies with larger sample numbers from more geospatially distinct populations and infection trials are indicated to further evaluate the possibility of flies as sentinels for antimicrobial resistance.
Identifiants
pubmed: 32105329
pii: 5762668
doi: 10.1093/femsec/fiaa027
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© FEMS 2020.