Electronic Cigarette Aerosol Modulates the Oral Microbiome and Increases Risk of Infection.
In Vitro Toxicology
Microbiome
Oral Microbiology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
27 Mar 2020
27 Mar 2020
Historique:
received:
27
11
2019
revised:
09
01
2020
accepted:
29
01
2020
pubmed:
28
2
2020
medline:
28
2
2020
entrez:
28
2
2020
Statut:
ppublish
Résumé
The trend of e-cigarette use among teens is ever increasing. Here we show the dysbiotic oral microbial ecology in e-cigarette users influencing the local host immune environment compared with non-smoker controls and cigarette smokers. Using 16S rRNA high-throughput sequencing, we evaluated 119 human participants, 40 in each of the three cohorts, and found significantly altered beta-diversity in e-cigarette users (p = 0.006) when compared with never smokers or tobacco cigarette smokers. The abundance of Porphyromonas and Veillonella (p = 0.008) was higher among vapers. Interleukin (IL)-6 and IL-1β were highly elevated in e-cigarette users when compared with non-users. Epithelial cell-exposed e-cigarette aerosols were more susceptible for infection. In vitro infection model of premalignant Leuk-1 and malignant cell lines exposed to e-cigarette aerosol and challenged by Porphyromonas gingivalis and Fusobacterium nucleatum resulted in elevated inflammatory response. Our findings for the first time demonstrate that e-cigarette users are more prone to infection.
Identifiants
pubmed: 32105635
pii: S2589-0042(20)30068-7
doi: 10.1016/j.isci.2020.100884
pmc: PMC7113564
pii:
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100884Subventions
Organisme : NIDA NIH HHS
ID : P30 DA011041
Pays : United States
Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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