Zygophyllum album saponins prevent atherogenic effect induced by deltamethrin via attenuating arterial accumulation of native and oxidized LDL in rats.
Animals
Aorta
/ drug effects
Atherosclerosis
/ immunology
CD36 Antigens
/ metabolism
Cholesterol
/ metabolism
Environmental Pollutants
/ toxicity
Lipid Metabolism
/ drug effects
Lipoproteins, LDL
/ blood
Liver
/ drug effects
Male
Nitriles
/ toxicity
Plant Leaves
/ chemistry
Pyrethrins
/ toxicity
Rats
Rats, Wistar
Receptors, LDL
/ metabolism
Saponins
/ isolation & purification
Tumor Necrosis Factor-alpha
/ metabolism
Zygophyllum
/ chemistry
Aorta
Atherogenic
Deltamethrin
LDL
Saponin
Zygophyllum album
Journal
Ecotoxicology and environmental safety
ISSN: 1090-2414
Titre abrégé: Ecotoxicol Environ Saf
Pays: Netherlands
ID NLM: 7805381
Informations de publication
Date de publication:
15 Apr 2020
15 Apr 2020
Historique:
received:
15
12
2019
revised:
06
02
2020
accepted:
07
02
2020
pubmed:
28
2
2020
medline:
28
5
2020
entrez:
28
2
2020
Statut:
ppublish
Résumé
The current study aimed to examine, for the first time, the relationship between exposure to deltamethrin (DLM) and atherogenic lipid profile disorders in adult Wistar rats, as well as, to verify the mechanism of the beneficial role of Zygophyllum album leaves extracts (ZALE). The experimental study was assessed using DLM (4 mg/kg b.w) either alone or co administered with ZALE (400 mg/kg b.w) orally for 90 days in rats. RP-HPLC-DAD-ESI-QTOF-MS was used to identify the bioactive metabolites present in ZALE. Plasmatic and aortic total cholesterol (TC), LDL-cholesterol (LDL-C), native LDL (LDL-apo B-100) and oxidized LDL (ox-LDL) were evaluated using auto-analyzer and a sandwich ELISA, respectively. The protein expressions of LDLR (native LDL receptor) and CD36 (Scavenger receptor class B) were evaluated in aorta or liver with a Western blot. The pathology has been confirmed with lipid stain (Oil Red O). Phytochemicals analysis revealed the presence of fifteen saponins in ZALE. Rats intoxicated with DLM revealed a significant increase in plasmatic and aortic lipid profile (TC, LDL-C, LDL-apo B-100 and ox-LDL), as well as, the concentration of the plasmatic cytokines include TNF-α, IL-2 and IL-6, compared to control. Hepatic native LDL and aortic CD36 receptor expression were increased in DLM treated group, however aortic LDL-R does not present any modification, when compared to control. The detected disturbances in lipid parameters were supported by Oil Red O applied. Due to their antioxidant activity, the bioactive compounds in ZALE as powerful agents able to prevent the pro-atherogenic effect observed in DLM-treated animals. These metabolites modulated most of inflammatory markers, prevented accumulation of lipid and lipoprotein biomarkers, regulated the major receptor regulators of hepatic cholesterol metabolism, as well as normalize lipid distribution in liver and aorta tissue.
Identifiants
pubmed: 32105945
pii: S0147-6513(20)30157-3
doi: 10.1016/j.ecoenv.2020.110318
pii:
doi:
Substances chimiques
CD36 Antigens
0
Environmental Pollutants
0
Lipoproteins, LDL
0
Nitriles
0
Pyrethrins
0
Receptors, LDL
0
Saponins
0
Tumor Necrosis Factor-alpha
0
oxidized low density lipoprotein
0
decamethrin
2JTS8R821G
Cholesterol
97C5T2UQ7J
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
110318Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.