The Molecular Anatomy of Mouse Skin during Hair Growth and Rest.
RNA-FISH
anagen
cell fate
differentiation trajectories
hair follicle
mouse skin
online skin atlas
single-cell transcriptomics
stem cells
telogen
Journal
Cell stem cell
ISSN: 1875-9777
Titre abrégé: Cell Stem Cell
Pays: United States
ID NLM: 101311472
Informations de publication
Date de publication:
05 03 2020
05 03 2020
Historique:
received:
02
05
2019
revised:
07
11
2019
accepted:
17
01
2020
pubmed:
29
2
2020
medline:
28
4
2021
entrez:
29
2
2020
Statut:
ppublish
Résumé
Skin homeostasis is orchestrated by dozens of cell types that together direct stem cell renewal, lineage commitment, and differentiation. Here, we use single-cell RNA sequencing and single-molecule RNA FISH to provide a systematic molecular atlas of full-thickness skin, determining gene expression profiles and spatial locations that define 56 cell types and states during hair growth and rest. These findings reveal how the outer root sheath (ORS) and inner hair follicle layers coordinate hair production. We found that the ORS is composed of two intermingling but transcriptionally distinct cell types with differing capacities for interactions with stromal cell types. Inner layer cells branch from transcriptionally uncommitted progenitors, and each lineage differentiation passes through an intermediate state. We also provide an online tool to explore this comprehensive skin cell atlas, including epithelial and stromal cells such as fibroblasts, vascular, and immune cells, to spur further discoveries in skin biology.
Identifiants
pubmed: 32109378
pii: S1934-5909(20)30012-6
doi: 10.1016/j.stem.2020.01.012
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
441-457.e7Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.