miR-9 Does Not Regulate Lamin A Expression in Metastatic Cells from Lung Adenocarcinoma.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
26 Feb 2020
Historique:
received: 17 01 2020
revised: 20 02 2020
accepted: 22 02 2020
entrez: 1 3 2020
pubmed: 1 3 2020
medline: 15 12 2020
Statut: epublish

Résumé

In lung adenocarcinoma, low lamin A expression in pleural metastatic cells has been proposed as a pejorative factor. miR-9 physiologically inhibits the expression of lamin A in neural cells and seems to be a central actor in the carcinogenesis and the metastatic process in lung cancer. Thus, it could be a good candidate to explain the reduction of lamin A expression in lung adenocarcinoma cells. miR-9 expression was analyzed in 16 pleural effusions containing metastatic cells from lung adenocarcinoma and was significantly reduced in patients from the 'Low lamin A expression' group compared to patients from the 'High lamin A expression' group. Then, carcinoma cells selection by fluorescence-activated cell sorting (FACS) was performed according to epithelial membrane antigen (EMA) expression, reflecting lamin A expression. miR-9 was underexpressed in lamin A- carcinoma cells compared to lamin A+ carcinoma cells in patients from the 'Low lamin A expression' group, whereas there was no difference of miR-9 expression between lamin A+ and lamin A- carcinoma cells in patients from the 'High lamin A expression' group. These results suggest that miR-9 does not regulate lamin A expression in metastatic cells from lung adenocarcinoma. On the contrary, miR-9 expression was shown to be reduced in lamin A-negative carcinoma cells.

Identifiants

pubmed: 32111074
pii: ijms21051599
doi: 10.3390/ijms21051599
pmc: PMC7084260
pii:
doi:

Substances chimiques

Lamin Type A 0
MIRN92 microRNA, human 0
MicroRNAs 0
Mucin-1 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : ARARD - Association Régionale d'Aide Respiratoire à Domicile) grant, Parc d'activités de Napollon, 100 avenue des Templiers, 13676 Aubagne Cedex, France
ID : ,

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Auteurs

Julien Guinde (J)

APHM, Hôpital Nord, Department of Thoracic Oncology-Pleural Diseases-Interventional Pulmonology, CEDEX 5, 13005 Marseille, France.

Audrey Benoit (A)

Aix Marseille Univ, INSERM, MMG, 13005 Marseille, France.

Diane Frankel (D)

Aix Marseille Univ, APHM, INSERM, MMG, Hôpital la Timone, Service de Biologie Cellulaire, 13005 Marseille, France.

Stéphane Robert (S)

Aix Marseille Univ, INSERM, C2VN, AMUTICYT Core facility, 13005 Marseille, France.

Kevin Ostacolo (K)

Aix Marseille Univ, INSERM, MMG, 13005 Marseille, France.
Department of Biochemistry and Molecular Biology, Biomedical Center, Faculty of Medicine, University of Iceland, 101 Reykjavik, Iceland.

Nicolas Lévy (N)

Aix Marseille Univ, APHM, INSERM, MMG, Hôpital la Timone, Département de Génétique Médicale, 13005 Marseille, France.

Philippe Astoul (P)

APHM, Hôpital Nord, Department of Thoracic Oncology-Pleural Diseases-Interventional Pulmonology, CEDEX 5, 13005 Marseille, France.

Patrice Roll (P)

Aix Marseille Univ, APHM, INSERM, MMG, Hôpital la Timone, Service de Biologie Cellulaire, 13005 Marseille, France.

Elise Kaspi (E)

Aix Marseille Univ, APHM, INSERM, MMG, Hôpital la Timone, Service de Biologie Cellulaire, 13005 Marseille, France.

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