Chronic whipworm infection exacerbates Schistosoma mansoni egg-induced hepatopathology in non-human primates.
Animal Diseases
/ parasitology
Animals
Chronic Disease
Coinfection
/ parasitology
Female
Granuloma
/ pathology
Humans
Liver
/ metabolism
Liver Diseases, Parasitic
/ parasitology
Male
Papio
Parasite Egg Count
Pilot Projects
Primates
Schistosoma mansoni
Schistosomiasis
/ parasitology
Transcriptome
Trichuriasis
/ parasitology
Trichuris
Co-infection
Helminths
Hepatopathology
NTDs
Schistosomiasis
Trichuriasis
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
28 Feb 2020
28 Feb 2020
Historique:
received:
07
11
2019
accepted:
17
02
2020
entrez:
1
3
2020
pubmed:
1
3
2020
medline:
21
10
2020
Statut:
epublish
Résumé
Schistosomiasis continues to inflict significant morbidity and mortality in the tropical and subtropical regions of the world. The disease endemicity overlaps with the transmission of other parasitic diseases. Despite the ubiquity of polyparasitism in tropical regions, particularly in rural communities, little is known about the impact of multiple helminth infections on disease progression. In this pilot study, we describe the influence of chronic Trichuris trichiura infection on Schistosoma mansoni egg-induced hepatopathology in infected baboons. Baboons with or without underlying whipworm infection were challenged with S. mansoni cercariae to establish schistosomiasis. Adult S. mansoni worms were recovered by perfusion and enumerated, hepatic granulomas were quantified via light microscopy, and transcriptional profiling of tissues were completed using RNA sequencing technologies. Co-infection with both S. mansoni and T. trichiura resulted in higher female schistosome worm burden and significantly larger liver granuloma sizes. Systems biology analyses of peripheral blood mononuclear cells (PBMC) revealed pathways associated with increased liver damage in co-infected baboons. Underlying chronic whipworm infection intensified schistosome egg-induced liver pathology in infected baboons. RNA-Seq analysis provided insight into pathways associated with increased liver damage, corroborating histological findings.
Sections du résumé
BACKGROUND
BACKGROUND
Schistosomiasis continues to inflict significant morbidity and mortality in the tropical and subtropical regions of the world. The disease endemicity overlaps with the transmission of other parasitic diseases. Despite the ubiquity of polyparasitism in tropical regions, particularly in rural communities, little is known about the impact of multiple helminth infections on disease progression. In this pilot study, we describe the influence of chronic Trichuris trichiura infection on Schistosoma mansoni egg-induced hepatopathology in infected baboons.
METHODS
METHODS
Baboons with or without underlying whipworm infection were challenged with S. mansoni cercariae to establish schistosomiasis. Adult S. mansoni worms were recovered by perfusion and enumerated, hepatic granulomas were quantified via light microscopy, and transcriptional profiling of tissues were completed using RNA sequencing technologies.
RESULTS
RESULTS
Co-infection with both S. mansoni and T. trichiura resulted in higher female schistosome worm burden and significantly larger liver granuloma sizes. Systems biology analyses of peripheral blood mononuclear cells (PBMC) revealed pathways associated with increased liver damage in co-infected baboons.
CONCLUSIONS
CONCLUSIONS
Underlying chronic whipworm infection intensified schistosome egg-induced liver pathology in infected baboons. RNA-Seq analysis provided insight into pathways associated with increased liver damage, corroborating histological findings.
Identifiants
pubmed: 32111243
doi: 10.1186/s13071-020-3980-z
pii: 10.1186/s13071-020-3980-z
pmc: PMC7048111
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109Subventions
Organisme : Bill and Melinda Gates Foundation
ID : OPP1097537
Organisme : National Institute of Allergy and Infectious Diseases
ID : R43/R44 AI103983
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