Microdialysis for the Assessment of Intervertebral Disc and Vertebral Cancellous Bone Metabolism in a Large Porcine Model.
Microdialysis
intervertebral disc
ischemia
metabolism
spine
vertebral cancellous bone
Journal
In vivo (Athens, Greece)
ISSN: 1791-7549
Titre abrégé: In Vivo
Pays: Greece
ID NLM: 8806809
Informations de publication
Date de publication:
Historique:
received:
17
12
2019
revised:
07
01
2020
accepted:
08
01
2020
entrez:
1
3
2020
pubmed:
1
3
2020
medline:
2
12
2020
Statut:
ppublish
Résumé
It remains challenging to evaluate the in vivo pathophysiological biochemical characteristics in spine tissue, due to lack of an applicable model and feasible methods. The aim of this study was to apply microdialysis for the assessment of basic metabolites from the C3-C4 intervertebral disc, C3 vertebral cancellous bone and subcutaneous adipose tissue in a large porcine model. In 7 pigs, glucose, pyruvate, lactate and glycerol concentrations were evaluated in an 8-hour sampling period. The mean lactate/pyruvate (L/P) ratios for the intervertebral disc and vertebral cancellous bone were comparable and exceeded the ischemic cut-off value of 25 for the entire sampling interval. For subcutaneous adipose tissue, the L/P ratio was below the ischemic cut-off. This exploratory study confirms previous findings of ischemia in bone and the intervertebral disc. This encourages new microdialysis study designs in spine tissue employing large porcine models to create new knowledge and a greater understanding of the metabolism and pathogenesis in spine tissue.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
It remains challenging to evaluate the in vivo pathophysiological biochemical characteristics in spine tissue, due to lack of an applicable model and feasible methods. The aim of this study was to apply microdialysis for the assessment of basic metabolites from the C3-C4 intervertebral disc, C3 vertebral cancellous bone and subcutaneous adipose tissue in a large porcine model.
MATERIALS AND METHODS
METHODS
In 7 pigs, glucose, pyruvate, lactate and glycerol concentrations were evaluated in an 8-hour sampling period.
RESULTS
RESULTS
The mean lactate/pyruvate (L/P) ratios for the intervertebral disc and vertebral cancellous bone were comparable and exceeded the ischemic cut-off value of 25 for the entire sampling interval. For subcutaneous adipose tissue, the L/P ratio was below the ischemic cut-off.
CONCLUSION
CONCLUSIONS
This exploratory study confirms previous findings of ischemia in bone and the intervertebral disc. This encourages new microdialysis study designs in spine tissue employing large porcine models to create new knowledge and a greater understanding of the metabolism and pathogenesis in spine tissue.
Identifiants
pubmed: 32111750
pii: 34/2/527
doi: 10.21873/invivo.11804
pmc: PMC7157874
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
527-532Informations de copyright
Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
Références
In Vivo. 2015 Sep-Oct;29(5):555-60
pubmed: 26359414
Acta Orthop. 2007 Feb;78(1):56-62
pubmed: 17453393
Osteoporos Int. 2012 Jul;23(7):1957-65
pubmed: 22086309
APMIS. 2019 Dec;127(12):779-788
pubmed: 31515843
Anticancer Res. 2019 Dec;39(12):6603-6620
pubmed: 31810926
Spine (Phila Pa 1976). 1998 Jan 1;23(1):1-7; discussion 8
pubmed: 9460145
J Bone Joint Surg Am. 2006 Apr;88 Suppl 2:21-4
pubmed: 16595438
Acta Orthop. 2011 Apr;82(2):241-5
pubmed: 21428845
BMJ. 2002 Mar 9;324(7337):588-91
pubmed: 11884326
Spine (Phila Pa 1976). 2004 Dec 1;29(23):2700-9
pubmed: 15564919
Acta Orthop. 2018 Dec;89(6):683-688
pubmed: 30080983
Curr Opin Rheumatol. 2005 Mar;17(2):134-40
pubmed: 15711224
BMC Musculoskelet Disord. 2015 Oct 29;16:326
pubmed: 26510621
In Vivo. 2010 May-Jun;24(3):257-64
pubmed: 20554996
Lab Anim. 2010 Apr;44(2):118-23
pubmed: 20357316
Intensive Care Med. 2015 Sep;41(9):1517-28
pubmed: 26194024
Arch Biochem Biophys. 2010 Nov 1;503(1):103-9
pubmed: 20655868
Curr Opin Rheumatol. 2007 Sep;19(5):457-62
pubmed: 17762611
Cancer Genomics Proteomics. 2010 Jul-Aug;7(4):191-205
pubmed: 20656985
Spine J. 2001 May-Jun;1(3):205-14
pubmed: 14588349
J Neurosurg. 2005 Mar;102(3):460-9
pubmed: 15796380
J Cell Physiol. 1996 Aug;168(2):424-32
pubmed: 8707878
Am J Pathol. 2004 Mar;164(3):915-24
pubmed: 14982845
Lab Anim. 2018 Dec;52(6):630-640
pubmed: 29653496
J Bone Joint Surg Am. 2006 Apr;88 Suppl 2:10-4
pubmed: 16595436
Curr Pharm Des. 2004;10(18):2145-52
pubmed: 15281890
Arch Orthop Trauma Surg. 2013 Dec;133(12):1735-45
pubmed: 24100766
Spine J. 2001 Jul-Aug;1(4):239-45
pubmed: 14588327
Clin Pharmacokinet. 2005;44(9):895-913
pubmed: 16122279
Vet Pathol. 2012 Mar;49(2):344-56
pubmed: 21441112
Spine J. 2016 Mar;16(3):432-8
pubmed: 26620946
J Intern Med. 1991 Oct;230(4):365-73
pubmed: 1919432
Spine J. 2004 Jan-Feb;4(1):88-98
pubmed: 14749197