Factors associated with positive outcomes of platelet-rich plasma therapy in Achilles tendinopathy.


Journal

European journal of orthopaedic surgery & traumatology : orthopedie traumatologie
ISSN: 1432-1068
Titre abrégé: Eur J Orthop Surg Traumatol
Pays: France
ID NLM: 9518037

Informations de publication

Date de publication:
Jul 2020
Historique:
received: 06 11 2019
accepted: 22 02 2020
pubmed: 1 3 2020
medline: 7 4 2021
entrez: 1 3 2020
Statut: ppublish

Résumé

The efficacy of platelet-rich plasma in the treatment for Achilles tendinopathy is debated. Therefore, it is important to know which factors, related to the subjects and/or the disease, are associated with positive or negative outcomes. Aim of this study was to evaluate in a large cohort of patients with Achilles mid-portion tendinopathy which variables were independently associated with a positive outcome after platelet-rich plasma treatment. Eighty-four subjects with Achilles tendinopathy were evaluated by means of VISA-A score and ultrasound and treated with a single platelet-rich plasma injection once a week for 3 weeks. Afterward, a rehabilitation program, based on eccentric training, was implemented. At 3 and 6 months, the relationship between the mean VISA-A score and the following putative predictors was evaluated: sex, age, physical activity, sport, smoking, metabolic risk factors, BMI, symptoms duration, tendon damage, neovessels, adherence to eccentric training. Finally, the percentage of clinically evident positive outcomes (defined as an increase in VISA-A score ≥ 20 points) related to each variable was computed. At final follow-up, using the General Linear Model for Repeated Measures procedure, male sex (0.02), age ≤ 40 (0.05) and adequate eccentric training (0.02) were found to be independently associated with a significant increase in the mean VISA-A score. Moreover, the clinically evident positive outcomes, as previously defined, were significantly associated with male sex (0.01), age ≤ 40 (0.000), BMI ≤ 25 (0.001), symptoms duration ≤ 12 months (0.02) and good adherence to eccentric training (0.004). Younger age, male sex and good adherence to eccentric training can be considered predictors of better results after platelet-rich plasma therapy in Achilles tendinopathy.

Sections du résumé

BACKGROUND BACKGROUND
The efficacy of platelet-rich plasma in the treatment for Achilles tendinopathy is debated. Therefore, it is important to know which factors, related to the subjects and/or the disease, are associated with positive or negative outcomes. Aim of this study was to evaluate in a large cohort of patients with Achilles mid-portion tendinopathy which variables were independently associated with a positive outcome after platelet-rich plasma treatment.
MATERIAL AND METHODS METHODS
Eighty-four subjects with Achilles tendinopathy were evaluated by means of VISA-A score and ultrasound and treated with a single platelet-rich plasma injection once a week for 3 weeks. Afterward, a rehabilitation program, based on eccentric training, was implemented. At 3 and 6 months, the relationship between the mean VISA-A score and the following putative predictors was evaluated: sex, age, physical activity, sport, smoking, metabolic risk factors, BMI, symptoms duration, tendon damage, neovessels, adherence to eccentric training. Finally, the percentage of clinically evident positive outcomes (defined as an increase in VISA-A score ≥ 20 points) related to each variable was computed.
RESULTS RESULTS
At final follow-up, using the General Linear Model for Repeated Measures procedure, male sex (0.02), age ≤ 40 (0.05) and adequate eccentric training (0.02) were found to be independently associated with a significant increase in the mean VISA-A score. Moreover, the clinically evident positive outcomes, as previously defined, were significantly associated with male sex (0.01), age ≤ 40 (0.000), BMI ≤ 25 (0.001), symptoms duration ≤ 12 months (0.02) and good adherence to eccentric training (0.004).
CONCLUSION CONCLUSIONS
Younger age, male sex and good adherence to eccentric training can be considered predictors of better results after platelet-rich plasma therapy in Achilles tendinopathy.

Identifiants

pubmed: 32112184
doi: 10.1007/s00590-020-02642-1
pii: 10.1007/s00590-020-02642-1
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

859-867

Commentaires et corrections

Type : CommentIn

Références

Abate M, Di Carlo L, Salini V (2019) Platelet rich plasma compared to dry needling in the treatment of non-insertional Achilles tendinopathy. Phys Sportsmed 47(2):232–237
pubmed: 30427252 doi: 10.1080/00913847.2018.1548886
Andia I, Maffulli N (2019) Some patients (and some of us) respond better to some biological therapies: the as yet unsolved conundrum. J Orthop Traumatol 20:1
Albano D, Messina C, Usuelli FG et al (2017) Magnetic resonance and ultrasound in Achilles tendinopathy: predictive role and response assessment to platelet-rich plasma and adipose-derived stromal vascular fraction injection. Eur J Radiol 95:130–135
pubmed: 28987658 doi: 10.1016/j.ejrad.2017.08.006
Alfredson H, Lorentzon R (2000) Chronic Achilles tendinosis: recommendations for treatment and prevention. Sports Med 29:135–146
pubmed: 10701715 doi: 10.2165/00007256-200029020-00005
Arnesen SM, Lawson MA (2006) Age-related changes in focal adhesions lead to altered cell behavior in tendon fibroblasts. Mech Ageing Dev 127:726–732
pubmed: 16780927 doi: 10.1016/j.mad.2006.05.003
Astill BD, Katsma MS, Cauthon DJ et al (2017) Sex-based difference in Achilles peritendinous levels of matrix metalloproteinases and growth factors after acute resistance exercise. J Appl Physiol 122:361–367
pubmed: 27881671 doi: 10.1152/japplphysiol.00878.2016
Bai MY, Chuang MH, Lin MF, Tang SL, Wong CC, Chan WP (2018) Relationships of age and sex with cytokine content and distribution in human platelet fibrin gels. Sci Rep 8(1):10642
pubmed: 30006555 pmcid: 6045593 doi: 10.1038/s41598-018-28376-z
Bell KJ, Fulcher ML, Rowlands DS, Kerse N (2013) Impact of autologous blood injections in treatment of mid-portion Achilles tendinopathy: double blind randomised controlled trial. BMJ 346:f2310
pubmed: 23599320 pmcid: 3629924 doi: 10.1136/bmj.f2310
Beyer R, Kongsgaard M, Hougs Kjær B et al (2015) Heavy slow resistance versus eccentric training as treatment for Achilles tendinopathy: a randomized controlled trial. Am J Sports Med 43:1704–1711
pubmed: 26018970 doi: 10.1177/0363546515584760
Birzniece V, Meinhardt UJ, Umpleby MA, Handelsman DJ, Ho KK (2011) Interaction between testosterone and growth hormone on whole-body protein anabolism occurs in the liver. J Clin Endocrinol Metab 96:1060–1067
pubmed: 21239519 doi: 10.1210/jc.2010-2521
Boesen AP, Dideriksen K, Couppé C et al (2014) Effect of growth hormone on aging connective tissue in muscle and tendon: gene expression, morphology, and function following immobilization and rehabilitation. J Appl Physiol 116:192–203
pubmed: 24235105 doi: 10.1152/japplphysiol.01077.2013
Boesen AP, Hansen R, Boesen MI et al (2017) Effect of high-volume injection, platelet-rich plasma, and sham treatment in chronic midportion Achilles tendinopathy: a randomized double-blinded prospective study. Am J Sports Med 45:2034–2043
pubmed: 28530451 doi: 10.1177/0363546517702862
Bongarzone S, Savickas V, Luzi F, Gee AD (2017) Targeting the receptor for advanced glycation endproducts (RAGE): a medicinal chemistry perspective. J Med Chem 60:7213–7232
pubmed: 28482155 pmcid: 5601361 doi: 10.1021/acs.jmedchem.7b00058
Brill KT, Weltman AL, Gentili A et al (2002) Single and combined effects of growth hormone and testosterone administration on measures of body composition, physical performance, mood, sexual function, bone turnover, and muscle gene expression in healthy older men. J Clin Endocrinol Metab 87:5649–5657
pubmed: 12466367 doi: 10.1210/jc.2002-020098
Cho HS, Song IH, Park SY et al (2011) Individual variation in growth factor concentrations in platelet-rich plasma and its influence on human mesenchymal stem cells. Korean J Lab Med 31:212–218
pubmed: 21779198 pmcid: 3129355 doi: 10.3343/kjlm.2011.31.3.212
Conde J, Gomez R, Bianco G et al (2011) Expanding the adipokine network in cartilage: identification and regulation of novel factors in human and murine chondrocytes. Ann Rheum Dis 70:551–559
pubmed: 21216818 doi: 10.1136/ard.2010.132399
Couppé C, Svensson RB, Silbernagel KG, Langberg H, Magnusson SP (2015) Eccentric or concentric exercises for the treatment of tendinopathies? J Orthop Sports Phys Ther 45:853–863
pubmed: 26471850 doi: 10.2519/jospt.2015.5910
Crescibene A, Napolitano M, Sbano R et al (2015) Infiltration of autologous growth factors in chronic tendinopathies. J Blood Transfus 2015:924380
pubmed: 26171277 pmcid: 4485529
de Jonge S, de Vos RJ, Weir A et al (2011) One-year follow-up of platelet-rich plasma treatment in chronic Achilles tendinopathy: a double-blind randomized placebo-controlled trial. Am J Sports Med 39:1623–1629
pubmed: 21602565 doi: 10.1177/0363546511404877
de Vos RJ, Weir A, van Schie HT et al (2010) Platelet-rich plasma injection for chronic Achilles tendinopathy: a randomized controlled trial. JAMA 303:144–149
pubmed: 20068208 doi: 10.1001/jama.2009.1986
Evanson JR, Guyton MK, Oliver DL et al (2014) Gender and age differences in growth factor concentrations from platelet-rich plasma in adults. Mil Med 179:799–805
pubmed: 25003868 doi: 10.7205/MILMED-D-13-00336
Faber M, Andersen MH, Sevel C et al (2015) The majority are not performing home-exercises correctly two weeks after their initial instruction-an assessor-blinded study. PeerJ 3:e1102. https://doi.org/10.7717/peerj.1102
doi: 10.7717/peerj.1102 pubmed: 26244112 pmcid: 4517955
Ferrero G, Fabbro E, Orlandi D et al (2012) Ultrasound-guided injection of platelet-rich plasma in chronic Achilles and patellar tendinopathy. J Ultrasound 15:260–266
pubmed: 23730392 pmcid: 3558233 doi: 10.1016/j.jus.2012.09.006
Filardo G, Kon E, Di Matteo B et al (2014) Platelet-rich plasma injections for the treatment of refractory Achilles tendinopathy: results at 4 years. Blood Transfus 12:533–540
pubmed: 24960641 pmcid: 4212034
Gaida JE, Alfredson H, Kiss ZS, Bass SL, Cook JL (2010) Asymptomatic Achilles tendon pathology is associated with a central fat distribution in men and a peripheral fat distribution in women: a cross sectional study of 298 individuals. BMC Musculoskelet Disord 11:41
pubmed: 20196870 pmcid: 2841085 doi: 10.1186/1471-2474-11-41
Gaida JE, Cook JL, Bass SL (2008) Adiposity and tendinopathy. Disabil Rehabil 30:1555–1562
pubmed: 18608380 doi: 10.1080/09638280701786864
Gaweda K, Tarczynska M, Krzyzanowski W (2010) Treatment of Achilles tendinopathy with platelet-rich plasma. Int J Sports Med 31:577–583
pubmed: 20535661 doi: 10.1055/s-0030-1255028
Gibney J, Wolthers T, Johannsson G, Umpleby AM, Ho KK (2005) Growth hormone and testosterone interact positively to enhance protein and energy metabolism in hypopituitary men. Am J Physiol Endocrinol Metab 289:E266–271
pubmed: 15727949 doi: 10.1152/ajpendo.00483.2004
Gómez LA, Escobar M, Peñuela O (2015) Standardization of a protocol for obtaining platelet rich plasma from blood donors; a tool for tissue regeneration procedures. Clin Lab 61:973–980
pubmed: 26427141 doi: 10.7754/Clin.Lab.2015.141141
Goode AP, Reiman MP, Harris L et al (2015) Eccentric training for prevention of hamstring injuries may depend on intervention compliance: a systematic review and meta-analysis. Br J Sports Med 49:349–356
pubmed: 25227125 doi: 10.1136/bjsports-2014-093466
Guelfi M, Pantalone A, Vanni D et al (2015) Long-term beneficial effects of platelet-rich plasma for non-insertional Achilles tendinopathy. Foot Ankle Surg 21:178–181
pubmed: 26235856 doi: 10.1016/j.fas.2014.11.005
Habets B, van Cingel RE (2015) Eccentric exercise training in chronic mid-portion Achilles tendinopathy: a systematic review on different protocols. Scand J Med Sci Sports 25:3–15
pubmed: 24650048 doi: 10.1111/sms.12208
Hansen M, Kjaer M (2016) Sex hormones and tendon. Adv Exp Med Biol 920:139–149
pubmed: 27535256 doi: 10.1007/978-3-319-33943-6_13
Hanisch K, Wedderkopp N (2019) Platelet-rich plasma (PRP) treatment of non-insertional Achilles tendinopathy in a two case series: no significant difference in effect between leukocyte-rich and leukocyte-poor PRP. Orthop Res Rev 11:55–60
pubmed: 31040726 pmcid: 6460817
Jian Z, Xiaolian M, Zhongmin S, Tanzhu L et al (2016) A prospective study of platelet-rich plasma as biological augmentation for acute Achilles tendon rupture repair. Biomed Res Int 2016:9364170
Keene DJ, Alsousou J, Harrison P et al (2019) PATH-2 trial group. Platelet rich plasma injection for acute Achilles tendon rupture: PATH-2 randomised, placebo controlled, superiority trial. BMJ 367:l6132
pubmed: 31748208 pmcid: 6863552 doi: 10.1136/bmj.l6132
Kıraç FS (2013) Is ethics approval necessary for all trials? A clear but not certain process. Mol Imaging Radionucl Ther 22:73–75
pubmed: 24416621 pmcid: 3888015 doi: 10.4274/Mirt.80664
Klindt J, Ford JJ, Macdonald GJ (1990) Synergism of testosterone propionate with growth hormone in promoting growth of hypophysectomized rats: effect of sexual differentiation. J Endocrinol 127:249–256
pubmed: 2250150 doi: 10.1677/joe.0.1270249
Knobloch K, Schreibmueller L, Longo UG, Vogt PM (2008) Eccentric exercises for the management of tendinopathy of the main body of the Achilles tendon with or without an AirHeel brace. A randomized controlled trial. B: effects of compliance. Disabil Rehabil 30:1692–1696
pubmed: 18720130 doi: 10.1080/09638280701785676
Kohler J, Popov C, Klotz B et al (2013) Uncovering the cellular and molecular changes in tendon stem/progenitor cells attributed to tendon aging and degeneration. Aging Cell 12:988–999
pubmed: 23826660 pmcid: 4225469 doi: 10.1111/acel.12124
Kostrominova TY, Brooks SV (2013) Age-related changes in structure and extracellular matrix protein expression levels in rat tendons. Age 35:2203–2214
pubmed: 23354684 pmcid: 3824999 doi: 10.1007/s11357-013-9514-2
Krogh TP, Ellingsen T, Christensen R, Jensen P, Fredberg U (2016) Ultrasound-guided injection therapy of Achilles tendinopathy with platelet-rich plasma or saline: a randomized, blinded, placebo-controlled trial. Am J Sports Med 44:1990–1997
pubmed: 27257167 doi: 10.1177/0363546516647958
Leifke E, Gorenoi V, Wichers C et al (2000) Age-related changes of serum sex hormones, insulin-like growth factor-1 and sex-hormone binding globulin levels in men: cross-sectional data from a healthy male cohort. Clin Endocrinol 53:689–695
doi: 10.1046/j.1365-2265.2000.01159.x
Lemoine JK, Lee JD, Trappe TA (2009) Impact of sex and chronic resistance training on human patellar tendon dry mass, collagen content, and collagen cross-linking. Am J Physiol Regul Integr Comp Physiol 296:R119–124
pubmed: 18945950 doi: 10.1152/ajpregu.90607.2008
Lin MT, Chiang CF, Wu CH et al (2018) Meta-analysis comparing autologous blood-derived products (including platelet-rich plasma) injection versus placebo in patients with Achilles tendinopathy. Arthroscopy 34(6):1966–1975.e5
pubmed: 29685839 doi: 10.1016/j.arthro.2018.01.030
Liu CJ, Yu KL, Bai JB, Tian DH, Liu GL (2019) Platelet-rich plasma injection for the treatment of chronic Achilles tendinopathy: a meta-analysis. Medicine 98:e15278
pubmed: 31008973 pmcid: 6494278 doi: 10.1097/MD.0000000000015278
Lohmann M, Walenda G, Hemeda H et al (2012) Donor age of human platelet lysate affects proliferation and differentiation of mesenchymal stem cells. PLoS One 7:e37839
pubmed: 22662236 pmcid: 3360602 doi: 10.1371/journal.pone.0037839
Macdermid JC, Silbernagel KG (2015) Outcome evaluation in tendinopathy: foundations of assessment and a summary of selected measures. J Orthop Sports Phys Ther 45:950–964
pubmed: 26471855 doi: 10.2519/jospt.2015.6054
Maffulli N, Longo UG, Testa V et al (2008) Italian translation of the VISA-A score for tendinopathy of the main body of the Achilles tendon. Disabil Rehabil 30:1635–1639
pubmed: 18608373 doi: 10.1080/09638280701785965
Magnusson SP, Hansen M, Langberg H et al (2007) The adaptability of tendon to loading differs in men and women. Int J Exp Pathol 88:237–240
pubmed: 17696904 doi: 10.1111/j.1365-2613.2007.00551.x
Malliaras P, Barton CJ, Reeves ND, Langberg H (2013) Achilles and patellar tendinopathy loading programmes: a systematic review comparing clinical outcomes and identifying potential mechanisms for effectiveness. Sports Med 43:267–286
pubmed: 23494258 doi: 10.1007/s40279-013-0019-z
Mauras N, Rini A, Welch S, Sager B, Murphy SP (2003) Synergistic effects of testosterone and growth hormone on protein metabolism and body composition in prepubertal boys. Metabolism 52:964–969
pubmed: 12898459 doi: 10.1016/S0026-0495(03)00163-X
Miller BF, Hansen M, Olesen JL et al (2007) Tendon collagen synthesis at rest and after exercise in women. J Appl Physiol 102:541–546
pubmed: 16990502 doi: 10.1152/japplphysiol.00797.2006
Morrison SM, Dick TJ, Wakeling JM (2015) Structural and mechanical properties of the human Achilles tendon: sex and strength effects. J Biomech 48:3530–3533
pubmed: 26159060 pmcid: 4592795 doi: 10.1016/j.jbiomech.2015.06.009
Murphy MC, Travers MJ, Chivers P et al (2019) Efficacy of heavy eccentric calf training for treating mid-portion Achilles tendinopathy: a systematic review and meta-analysis. Br J Sports Med 53:1070–1077
pubmed: 30636702 doi: 10.1136/bjsports-2018-099934
Ohberg L, Lorentzon R, Alfredson H (2001) Neovascularisation in Achilles tendons with painful tendinosis but not in normal tendons: an ultrasonographic investigation. Knee Surg Sports Traumatol Arthrosc 9:233–238
pubmed: 11522081 doi: 10.1007/s001670000189
Pham T, van der Heijde D, Altman RD et al (2004) OMERACT-OARSI initiative: Osteoarthritis Research Society International set of responder criteria for osteoarthritis clinical trials revisited. Osteoarthr Cartil 12:389–399
pubmed: 15094138 pmcid: 15094138 doi: 10.1016/j.joca.2004.02.001
Ram R, Meeuwisse W, Patel C, Wiseman DA, Wiley JP (2013) The limited effectiveness of a home-based eccentric training for treatment of Achilles tendinopathy. Clin Investig Med 36:E197–E206
doi: 10.25011/cim.v36i4.19953
Russo V, Mauro A, Martelli A et al (2015) Cellular and molecular maturation in fetal and adult ovine calcaneal tendons. J Anat 226:126–142
pubmed: 25546075 doi: 10.1111/joa.12269
Ruzzini L, Abbruzzese F, Rainer A et al (2014) Characterization of age-related changes of tendon stem cells from adult human tendons. Knee Surg Sports Traumatol Arthrosc 22:2856–2866
pubmed: 23503946 doi: 10.1007/s00167-013-2457-4
Salini V, Vanni D, Pantalone A, Abate M (2015) platelet rich plasma therapy in non-insertional Achilles tendinopathy: the efficacy is reduced in 60-years old people compared to young and middle-age individuals. Front Aging Neurosci 7:228
pubmed: 26696880 pmcid: 4674567 doi: 10.3389/fnagi.2015.00228
Scott SH, Winter DA (1990) Internal forces of chronic running injury sites. Med Sci Sports Exerc 22:357–369
pubmed: 2381304 doi: 10.1249/00005768-199006000-00013
Sullivan BE, Carroll CC, Jemiolo B et al (1985) (2009) Effect of acute resistance exercise and sex on human patellar tendon structural and regulatory mRNA expression. J Appl Physiol 106:468–475
doi: 10.1152/japplphysiol.91341.2008
Sunding K, Fahlström M, Werner S, Forssblad M, Willberg L (2016) Evaluation of Achilles and patellar tendinopathy with greyscale ultrasound and colour Doppler: using a four-grade scale. Knee Surg Sports Traumatol Arthrosc 24:1988–1996
pubmed: 25193569 doi: 10.1007/s00167-014-3270-4
Taniguchi Y, Yoshioka T, Sugaya H et al (2019) Growth factor levels in leukocyte-poor platelet-rich plasma and correlations with donor age, gender, and platelets in the Japanese population. J Exp Orthop 6:4
pubmed: 30712144 pmcid: 6359998 doi: 10.1186/s40634-019-0175-7
Tian J, Cheng LH, Cui X et al (2019) Investigating the effect of age on platelet ultrastructure using transmission electron microscopy. Int Wound J 16(6):1457–1463
pubmed: 31486290 doi: 10.1111/iwj.13214
Tian J, Lei XX, Xuan L et al (2019) The effects of aging, diabetes mellitus, and antiplatelet drugs on growth factors and anti-aging proteins in platelet-rich plasma. Platelets 30(6):773–792
pubmed: 30252623 doi: 10.1080/09537104.2018.1514110
Torricelli P, Veronesi F, Pagani S et al (2013) In vitro tenocyte metabolism in aging and oestrogen deficiency. Age 35:2125–2136
pubmed: 23274854 doi: 10.1007/s11357-012-9500-0
Tsai WC, Chang HN, Yu TY et al (2011) Decreased proliferation of aging tenocytes is associated with down-regulation of cellular senescence-inhibited gene and up-regulation of p27. J Orthop Res 29:1598–1603
pubmed: 21452304 doi: 10.1002/jor.21418
Volpi P, Quaglia A, Schoenhuber H et al (2010) Growth factors in the management of sport-induced tendinopathies: results after 24 months from treatment. A pilot study. J Sports Med Phys Fitness 50:494–500
pubmed: 21178936
von Wehren L, Pokorny K, Blanke F et al (2019) Injection with autologous conditioned serum has better clinical results than eccentric training for chronic Achilles tendinopathy. Knee Surg Sports Traumatol Arthrosc 27(9):2744–2753
doi: 10.1007/s00167-019-05465-8
Wang Y, Han C, Hao J, Ren Y, Wang J (2019) Efficacy of platelet-rich plasma injections for treating Achilles tendonitis: systematic review of high-quality randomized controlled trials. Orthopade 48:784–791
pubmed: 30937491 doi: 10.1007/s00132-019-03711-y
Xiong G, Lingampalli N, Koltsov JCB et al (2018) Men and women differ in the biochemical composition of platelet-rich plasma. Am J Sports Med 46:409–419
pubmed: 29211968 doi: 10.1177/0363546517740845
Yoshizawa A, Clemmons DR (2000) Testosterone and insulin-like growth factor (IGF) I interact in controlling IGF-binding protein production in androgen-responsive foreskin fibroblasts. J Clin Endocrinol Metab 85:1627–1633
pubmed: 10770208
Zhou Z, Akinbiyi T, Xu L et al (2010) Tendon-derived stem/progenitor cell aging: defective self-renewal and altered fate. Aging Cell 9:911–915
pubmed: 20569237 pmcid: 2944918 doi: 10.1111/j.1474-9726.2010.00598.x

Auteurs

Michele Abate (M)

Department of Medicine and Science of Aging, University G. D'Annunzio, Chieti-Pescara, Via dei Vestini 31, 66013, Chieti Scalo, CH, Italy. m.abate@unich.it.

Luigi Di Carlo (L)

Department of Medicine and Science of Aging, University G. D'Annunzio, Chieti-Pescara, Via dei Vestini 31, 66013, Chieti Scalo, CH, Italy.

Alberto Belluati (A)

Division of Orthopedics and Traumatology, Santa Maria Delle Croci Hospital, Ravenna, Italy.

Vincenzo Salini (V)

Division of Orthopedics and Traumatology, San Raffaele Hospital, Milan, Italy.

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