Glucose homeostasis is impaired in mice deficient in the neuropeptide 26RFa (QRFP).
Animals
Blood Glucose
/ metabolism
Energy Metabolism
/ genetics
Feeding Behavior
Gene Knockout Techniques
Homeostasis
/ genetics
Hyperglycemia
/ genetics
Insulin
/ biosynthesis
Insulin-Secreting Cells
/ metabolism
Locomotion
/ genetics
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Neuropeptides
/ deficiency
Phenotype
glucose-insulin homeostasis
incretin
insulin secretion
islet beta cell
Journal
BMJ open diabetes research & care
ISSN: 2052-4897
Titre abrégé: BMJ Open Diabetes Res Care
Pays: England
ID NLM: 101641391
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
09
10
2019
revised:
09
01
2020
accepted:
28
01
2020
entrez:
2
3
2020
pubmed:
3
3
2020
medline:
8
1
2021
Statut:
ppublish
Résumé
26RFa (pyroglutamyl RFamide peptide (QRFP)) is a biologically active peptide that has been found to control feeding behavior by stimulating food intake, and to regulate glucose homeostasis by acting as an incretin. The aim of the present study was thus to investigate the impact of 26RFa gene knockout on the regulation of energy and glucose metabolism. 26RFa mutant mice were generated by homologous recombination, in which the entire coding region of prepro26RFa was replaced by the iCre sequence. Energy and glucose metabolism was evaluated through measurement of complementary parameters. Morphological and physiological alterations of the pancreatic islets were also investigated. Our data do not reveal significant alteration of energy metabolism in the 26RFa-deficient mice except the occurrence of an increased basal metabolic rate. By contrast, 26RFa mutant mice exhibited an altered glycemic phenotype with an increased hyperglycemia after a glucose challenge associated with an impaired insulin production, and an elevated hepatic glucose production. Two-dimensional and three-dimensional immunohistochemical experiments indicate that the insulin content of pancreatic β cells is much lower in the 26RFa Disruption of the 26RFa gene induces substantial alteration in the regulation of glucose homeostasis, with in particular a deficit in insulin production by the pancreatic islets. These findings further support the notion that 26RFa is an important regulator of glucose homeostasis.
Identifiants
pubmed: 32114486
pii: 8/1/e000942
doi: 10.1136/bmjdrc-2019-000942
pmc: PMC7050347
pii:
doi:
Substances chimiques
Blood Glucose
0
Insulin
0
Neuropeptides
0
RFamide peptide
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.
Déclaration de conflit d'intérêts
Competing interests: None declared.
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