Functional and Metabolic Characterization of Microglia Culture in a Defined Medium.
culture
metabolic reprogramming
microglia
motility
phagocytosis
purinergic receptors
ramification
Journal
Frontiers in cellular neuroscience
ISSN: 1662-5102
Titre abrégé: Front Cell Neurosci
Pays: Switzerland
ID NLM: 101477935
Informations de publication
Date de publication:
2020
2020
Historique:
received:
21
10
2019
accepted:
24
01
2020
entrez:
3
3
2020
pubmed:
3
3
2020
medline:
3
3
2020
Statut:
epublish
Résumé
Microglia are the endogenous immune cells of the brain and act as sensor of infection and pathologic injury to the brain, leading to a rapid plastic process of activation that culminates in the endocytosis and phagocytosis of damaged tissue. Microglia cells are the most plastic cells in the brain. Microglia isolation from their environment as well as culturing them in the presence of serum alter their function and lead to a rapid loss of their signature gene expression. Previous studies have identified pivotal factors allowing microglia culture in the absence of serum. Here, we have further characterized the function, expression of markers, metabolic status and response to pro and anti-inflammatory stimulus of microglia isolated by magnetic-activated cell sorting and cultured in a chemically defined medium. We have compared this new method with previous traditional protocols of culturing microglia that use high concentrations of serum.
Identifiants
pubmed: 32116565
doi: 10.3389/fncel.2020.00022
pmc: PMC7025516
doi:
Types de publication
Journal Article
Langues
eng
Pagination
22Informations de copyright
Copyright © 2020 Montilla, Zabala, Matute and Domercq.
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