Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations.
AAV (adeno-associated virus)
circuit tracing
monosynaptic tracing
rabies
virus
Journal
Frontiers in synaptic neuroscience
ISSN: 1663-3563
Titre abrégé: Front Synaptic Neurosci
Pays: Switzerland
ID NLM: 101548972
Informations de publication
Date de publication:
2020
2020
Historique:
received:
20
08
2019
accepted:
23
01
2020
entrez:
3
3
2020
pubmed:
3
3
2020
medline:
3
3
2020
Statut:
epublish
Résumé
Monosynaptically-restricted transsynaptic tracing using deletion-mutant rabies virus (RV) has become a widely used technique in neuroscience, allowing identification, imaging, and manipulation of neurons directly presynaptic to a starting neuronal population. Its most common implementation is to use Cre mouse lines in combination with Cre-dependent "helper" adeno-associated viral vectors (AAVs) to supply the required genes to the targeted population before subsequent injection of a first-generation (ΔG) rabies viral vector. Here we show that the efficiency of transsynaptic spread and the degree of nonspecific labeling in wild-type control animals depend strongly on the concentrations of these helper AAVs. Our results suggest practical guidelines for achieving good results.
Identifiants
pubmed: 32116642
doi: 10.3389/fnsyn.2020.00006
pmc: PMC7033752
doi:
Types de publication
Journal Article
Langues
eng
Pagination
6Subventions
Organisme : NIMH NIH HHS
ID : RF1 MH120017
Pays : United States
Informations de copyright
Copyright © 2020 Lavin, Jin, Lea and Wickersham.
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