Metabolic Implications of Using BioOrthogonal Non-Canonical Amino Acid Tagging (BONCAT) for Tracking Protein Synthesis.
BONCAT
L-azidohomoalanine
L-homopropargylglycine
metabolomics
non-canonical amino acids
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2020
2020
Historique:
received:
07
12
2019
accepted:
28
01
2020
entrez:
3
3
2020
pubmed:
3
3
2020
medline:
3
3
2020
Statut:
epublish
Résumé
BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) is a powerful tool for tracking protein synthesis on the level of single cells within communities and whole organisms. A basic premise of BONCAT is that the non-canonical amino acids (NCAA) used to track translational activity do not significantly alter cellular physiology. If the NCAA would induce changes in the metabolic state of cells, interpretation of BONCAT studies could be challenging. To address this knowledge-gap, we have used a global metabolomics analyses to assess the intracellular effects of NCAA incorporation. Two NCAA were tested:
Identifiants
pubmed: 32117186
doi: 10.3389/fmicb.2020.00197
pmc: PMC7031258
doi:
Types de publication
Journal Article
Langues
eng
Pagination
197Informations de copyright
Copyright © 2020 Steward, Eilers, Tripet, Fuchs, Dorle, Rawle, Soriano, Balasubramanian, Copié, Bothner and Hatzenpichler.
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