Influence of the Fabrication Accuracy of Hot-Embossed PCL Scaffolds on Cell Growths.
cell viability
demolding force
hot embossing
microstructured scaffolds
polycaprolactone
Journal
Frontiers in bioengineering and biotechnology
ISSN: 2296-4185
Titre abrégé: Front Bioeng Biotechnol
Pays: Switzerland
ID NLM: 101632513
Informations de publication
Date de publication:
2020
2020
Historique:
received:
29
08
2019
accepted:
29
01
2020
entrez:
3
3
2020
pubmed:
3
3
2020
medline:
3
3
2020
Statut:
epublish
Résumé
Polycaprolactone (PCL) is a biocompatible and biodegradable polymer widely used for the realization of 3D scaffold for tissue engineering applications. The hot embossing technique (HE) allows the obtainment of PCL scaffolds with a regular array of micro pillars on their surface. The main drawback affecting this kind of micro fabrication process is that such structural superficial details can be damaged when detaching the replica from the mold. Therefore, the present study has focused on the optimization of the HE processes through the development of an analytical model for the prediction of the demolding force as a function of temperature. This model allowed calculating the minimum demolding force to obtain regular micropillars without defects. We demonstrated that the results obtained by the analytical model agree with the experimental data. To address the importance of controlling accurately the fabricated microstructures, we seeded on the PCL scaffolds human stromal cell line (HS-5) and monocytic leukemia cell line (THP-1) to evaluate how the presence of regular or deformed pillars affect cells viability.
Identifiants
pubmed: 32117950
doi: 10.3389/fbioe.2020.00084
pmc: PMC7033415
doi:
Types de publication
Journal Article
Langues
eng
Pagination
84Informations de copyright
Copyright © 2020 Limongi, Dattola, Botta, Coluccio, Candeloro, Cucè, Scopacasa, Gallo Cantafio, Critello, Pullano, Fiorillo, Tagliaferri, Tassone, Lamanna, Di Fabrizio and Perozziello.
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