Alternatively Constructed Estrogen Receptor Alpha-Driven Super-Enhancers Result in Similar Gene Expression in Breast and Endometrial Cell Lines.
Basic Helix-Loop-Helix Transcription Factors
/ genetics
Binding Sites
/ genetics
Breast Neoplasms
/ genetics
Cell Line, Tumor
Chromatin Immunoprecipitation Sequencing
DNA-Binding Proteins
/ genetics
E-Box Elements
/ genetics
Endometrial Neoplasms
/ genetics
Endometrium
/ cytology
Enhancer Elements, Genetic
Estrogen Receptor alpha
/ genetics
Female
Forkhead Box Protein M1
/ genetics
Gene Expression Regulation, Neoplastic
/ genetics
Humans
MCF-7 Cells
Muscle Proteins
/ genetics
TEA Domain Transcription Factors
Transcription Factors
/ genetics
Transcriptome
ChIP-seq
Ishikawa cell line
MCF-7 cell line
enhancer
estrogen receptor alpha (ERα), transcription factor
super-enhancer
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
27 Feb 2020
27 Feb 2020
Historique:
received:
22
01
2020
revised:
21
02
2020
accepted:
25
02
2020
entrez:
4
3
2020
pubmed:
4
3
2020
medline:
29
12
2020
Statut:
epublish
Résumé
Super-enhancers (SEs) are clusters of highly active enhancers, regulating cell type-specific and disease-related genes, including oncogenes. The individual regulatory regions within SEs might be simultaneously bound by different transcription factors (TFs) and co-regulators, which together establish a chromatin environment conducting to effective transcription. While cells with distinct TF profiles can have different functions, how different cells control overlapping genetic programs remains a question. In this paper, we show that the construction of estrogen receptor alpha-driven SEs is tissue-specific, both collaborating TFs and the active SE components greatly differ between human breast cancer-derived MCF-7 and endometrial cancer-derived Ishikawa cells; nonetheless, SEs common to both cell lines have similar transcriptional outputs. These results delineate that despite the existence of a combinatorial code allowing alternative SE construction, a single master regulator might be able to determine the overall activity of SEs.
Identifiants
pubmed: 32120995
pii: ijms21051630
doi: 10.3390/ijms21051630
pmc: PMC7084573
pii:
doi:
Substances chimiques
Basic Helix-Loop-Helix Transcription Factors
0
DNA-Binding Proteins
0
ESR1 protein, human
0
Estrogen Receptor alpha
0
FOXM1 protein, human
0
Forkhead Box Protein M1
0
Muscle Proteins
0
TEA Domain Transcription Factors
0
TEAD4 protein, human
0
Transcription Factors
0
TCF12 protein, human
142661-93-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : MOLMEDEX FUN-OMICS
ID : GINOP-2.3.3-15-2016-00007
Organisme : Debrecen Venture Catapult Program
ID : EFOP-3.6.1-16-2016-00022
Organisme : Hungarian Scientific Research Fund
ID : K 129166
Organisme : New National Excellence Program of the Ministry of Human Capacities
ID : ÚNKP-17-3-IV-DE-140
Organisme : New National Excellence Program of the Ministry of Human Capacities
ID : ÚNKP-18-3-III-DE-253
Organisme : Hungarian Scientific Research Fund
ID : PD 124843
Organisme : New National Excellence Program of the Ministry of Human Capacities
ID : ÚNKP-19-4-DE-173
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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