Towards PCB-Based Miniaturized Thermocyclers for DNA Amplification.
genomic Salmonella DNA
micro polymerase chain reaction (microPCR)
microfluidics
multi-well PCR
printed circuit board (PCB)
simulation
static
Journal
Micromachines
ISSN: 2072-666X
Titre abrégé: Micromachines (Basel)
Pays: Switzerland
ID NLM: 101640903
Informations de publication
Date de publication:
28 Feb 2020
28 Feb 2020
Historique:
received:
04
02
2020
revised:
24
02
2020
accepted:
25
02
2020
entrez:
4
3
2020
pubmed:
4
3
2020
medline:
4
3
2020
Statut:
epublish
Résumé
In recent years, printed circuit board (PCB)-based microfluidics have been explored as a means to achieve standardization, seamless integration, and large-scale manufacturing of microfluidics, thus paving the way for widespread commercialization of developed prototypes. In this work, static micro polymerase chain reaction (microPCR) devices comprising resistive microheaters integrated on PCBs are introduced as miniaturized thermocyclers for efficient DNA amplification. Their performance is compared to that of conventional thermocyclers, in terms of amplification efficiency, power consumption and duration. Exhibiting similar efficiency to conventional thermocyclers, PCB-based miniaturized thermocycling achieves faster DNA amplification, with significantly smaller power consumption. Simulations guide the design of such devices and propose means for further improvement of their performance.
Identifiants
pubmed: 32121172
pii: mi11030258
doi: 10.3390/mi11030258
pmc: PMC7143664
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : European Commission
ID : 68768
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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