Lifestyle intervention in individuals with impaired glucose regulation affects Caveolin-1 expression and DNA methylation.


Journal

Adipocyte
ISSN: 2162-397X
Titre abrégé: Adipocyte
Pays: United States
ID NLM: 101567863

Informations de publication

Date de publication:
12 2020
Historique:
entrez: 4 3 2020
pubmed: 4 3 2020
medline: 24 2 2021
Statut: ppublish

Résumé

We investigated whether a lifestyle intervention could influence expression and DNA methylation of diabetes-related genes in patients with impaired glucose regulation (IGR), the results were compared to bariatric surgery, considering it an intensive change. Twenty participants with IGR had adipose tissue biopsy and blood collected pre- and post-lifestyle (6 months) intervention; 12 obese patients had subcutaneous fat taken before and after bariatric surgery. RNA/DNA was extracted from all samples and underwent qPCR. DNA was bisulphite converted and 12 CpG sites of Caveolin-1 ( lifestyle intervention resulted in opposite direction changes in fat tissue and blood for Our findings suggest a role for

Identifiants

pubmed: 32125224
doi: 10.1080/21623945.2020.1732513
pmc: PMC7153542
doi:

Substances chimiques

CAV1 protein, human 0
Caveolin 1 0
DNA 9007-49-2
Glucose IY9XDZ35W2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

96-107

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Auteurs

Helene A Fachim (HA)

Faculty of Biology, Medicine and Health and Manchester Academic Health Sciences Centre, University of Manchester, Manchester, UK.
Department of Diabetes and Endocrinology, Salford Royal NHS Foundation Trust, Salford, UK.

Kirk Siddals (K)

Faculty of Biology, Medicine and Health and Manchester Academic Health Sciences Centre, University of Manchester, Manchester, UK.
Department of Diabetes and Endocrinology, Salford Royal NHS Foundation Trust, Salford, UK.

Nagaraj Malipatil (N)

Faculty of Biology, Medicine and Health and Manchester Academic Health Sciences Centre, University of Manchester, Manchester, UK.
Department of Diabetes and Endocrinology, Salford Royal NHS Foundation Trust, Salford, UK.

Rachelle P Donn (RP)

Faculty of Biology, Medicine and Health and Manchester Academic Health Sciences Centre, University of Manchester, Manchester, UK.

Gabriela Yc Moreno (GY)

Dirección General de Calidad y Educación en Salud, Secretaría de Salud, Mexico City, Mexico.

Caroline F Dalton (CF)

Biomolecular Science Research Centre, Sheffield Hallam University, Sheffield, UK.

Safwaan Adam (S)

Faculty of Biology, Medicine and Health and Manchester Academic Health Sciences Centre, University of Manchester, Manchester, UK.
Department of Endocrinology, The Christie NHS Foundation Trust, Manchester, UK.
Department of Endocrinology, Diabetes and Metabolism, Manchester Royal Infirmary, Manchester, UK.

Handrean Soran (H)

Department of Endocrinology, Diabetes and Metabolism, Manchester Royal Infirmary, Manchester, UK.
Lipoprotein Research Group, Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine & Health, University of Manchester, Core Technology Facility, Manchester, UK.

J Martin Gibson (JM)

Faculty of Biology, Medicine and Health and Manchester Academic Health Sciences Centre, University of Manchester, Manchester, UK.
Department of Diabetes and Endocrinology, Salford Royal NHS Foundation Trust, Salford, UK.

Adrian H Heald (AH)

Faculty of Biology, Medicine and Health and Manchester Academic Health Sciences Centre, University of Manchester, Manchester, UK.
Department of Diabetes and Endocrinology, Salford Royal NHS Foundation Trust, Salford, UK.

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