New approach for membrane protein reconstitution into peptidiscs and basis for their adaptability to different proteins.
Cryo-electron microscopy
E. coli
Peptidiscs
biochemistry
chemical biology
membrane mimetics
molecular biophysics
nanodiscs
structural biology
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
03 03 2020
03 03 2020
Historique:
received:
21
11
2019
accepted:
24
02
2020
entrez:
4
3
2020
pubmed:
4
3
2020
medline:
5
5
2021
Statut:
epublish
Résumé
Previously we introduced peptidiscs as an alternative to detergents to stabilize membrane proteins in solution (Carlson et al., 2018). Here, we present 'on-gradient' reconstitution, a new gentle approach for the reconstitution of labile membrane-protein complexes, and used it to reconstitute
Identifiants
pubmed: 32125274
doi: 10.7554/eLife.53530
pii: 53530
pmc: PMC7053995
doi:
pii:
Substances chimiques
Bacterial Proteins
0
Membrane Proteins
0
Banques de données
PDB
['6UZH', '6UZ2', '6UZL']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Natural Sciences and Engineering Research Council of Canada
ID : RGPIN-2016-04241
Informations de copyright
© 2020, Angiulli et al.
Déclaration de conflit d'intérêts
GA, HD, HS, IW, TW No competing interests declared, FD is the scientific founder of Peptidisc Biotech
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