Characterization of the IgA response to PRRS virus in pig oral fluids.
Animals
Antibodies, Viral
/ analysis
Body Fluids
/ immunology
Cells, Cultured
Enzyme-Linked Immunosorbent Assay
Female
Immunity, Mucosal
/ physiology
Immunoglobulin A
/ analysis
Macrophages, Alveolar
/ immunology
Mouth
/ immunology
Mouth Mucosa
/ immunology
Porcine Reproductive and Respiratory Syndrome
/ immunology
Porcine respiratory and reproductive syndrome virus
/ genetics
Real-Time Polymerase Chain Reaction
Swine
Viral Proteins
/ genetics
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
31
10
2019
accepted:
28
01
2020
entrez:
4
3
2020
pubmed:
4
3
2020
medline:
18
6
2020
Statut:
epublish
Résumé
Porcine Reproductive and Respiratory Syndrome (PRRS) is a complex model of host/virus relationship. Disease control measures often includes "acclimatization", i.e. the exposure of PRRS-naïve gilts and sows to PRRSV-infected pigs and premises before the breeding period. In this respect, we had repeatedly observed an association between PRRSV-specific IgA responses in oral fluids (OF) of gilts and block of PRRSV spread. Therefore, we set out to investigate in vitro the inhibition of PRRSV replication by OF samples with different titers of PRRSV-specific IgA and IgG antibody, using Real-time RT PCR. PRRSV yield reduction in monocyte-derived macrophages was associated with the IgA content in OF samples, whereas the IgG-rich samples were sometimes associated with antibody-dependent enhancement (ADE) of replication. Accordingly, we could discriminate between ADE-positive and ADE-negative PRRSV strains. Next, we separated Ig isotypes in OF samples of PRRSV-infected pigs by means of protein A and size exclusion chromatography. The above results were confirmed by using separated Ig isotypes. Both dimeric and monomeric IgA were associated with the strongest reduction of PRRSV replication. The treatment of pig macrophages with separated OF antibodies before PRRSV infection was also associated with PRRSV yield reduction, along with clear changes of both CD163 and CD169 surface expression. Our results point at a role of mucosal IgA in the control of PRRSV replication by extra- and/or intracellular interaction with PRRSV, as well as by induction of signals leading to a reduced susceptibility of macrophages to PRRSV infection.
Identifiants
pubmed: 32126095
doi: 10.1371/journal.pone.0229065
pii: PONE-D-19-30365
pmc: PMC7053757
doi:
Substances chimiques
Antibodies, Viral
0
Immunoglobulin A
0
Viral Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0229065Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Virology. 2002 Oct 10;302(1):9-20
pubmed: 12429512
Front Microbiol. 2016 Jun 02;7:832
pubmed: 27313573
J Gen Virol. 1994 May;75 ( Pt 5):1071-81
pubmed: 8176369
Clin Vaccine Immunol. 2007 Mar;14(3):269-75
pubmed: 17215336
Vet Res. 2012 Apr 19;43:30
pubmed: 22515169
Vet Microbiol. 1997 Apr;55(1-4):277-87
pubmed: 9220623
Tierarztl Prax Ausg G Grosstiere Nutztiere. 2011;39(2):101-12
pubmed: 22138772
Virus Res. 2010 Dec;154(1-2):185-92
pubmed: 20837071
Exp Cell Res. 1977 Feb;104(2):255-62
pubmed: 65285
BMC Vet Res. 2010 Jun 04;6:30
pubmed: 20525333
J Anim Sci. 2005 Jul;83(7):1494-502
pubmed: 15956456
J Gen Virol. 2006 Jul;87(Pt 7):1835-41
pubmed: 16760385
Arch Virol. 2000;145(4):659-88
pubmed: 10893147
Vet J. 2008 Sep;177(3):345-51
pubmed: 17644436
Curr Top Microbiol Immunol. 2014;382:221-35
pubmed: 25116102
Virol J. 2009 Oct 27;6:177
pubmed: 19860914
Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):6901-5
pubmed: 1323121
J Virol. 1999 Jan;73(1):270-80
pubmed: 9847330
Vet Res. 2010 Sep-Oct;41(5):74
pubmed: 20663475
J Vet Diagn Invest. 1994 Jul;6(3):289-92
pubmed: 7948196
Virus Res. 2010 Dec;154(1-2):98-103
pubmed: 20655964
Vet Microbiol. 2013 Apr 12;163(1-2):13-22
pubmed: 23313323
Am J Respir Crit Care Med. 1996 Nov;154(5):1261-6
pubmed: 8912733
BMC Vet Res. 2013 Mar 28;9:61
pubmed: 23537175
J Gen Virol. 2000 May;81(Pt 5):1327-34
pubmed: 10769076
Vet Sci. 2019 Mar 11;6(1):
pubmed: 30862035
Vet Immunol Immunopathol. 2018 Jun;200:32-39
pubmed: 29776610